C Bai1, J Pan, X Li. 1. PUMC Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100730.
Abstract
OBJECTIVE: To evaluate the incidence of factor V Leiden and prothrombin gene G20210A mutation in patients with venous thrombosis and healthy volunteers. METHODS: Factor V Leiden and prothrombin gene G20210A mutation were analysed in 97 cases of venous thrombosis and 100 healthy volunteers with the methods of one-step PCR-RFLP. RESULTS: PCR products for the factor V gene (175 bp) and for the prothrombin gene (118 bp) were identified to 157 bp and 98 bp fragments by electrophoresis after Taq I treatment. No factor V Leiden and prothrombin gene G20210A mutation were found in either group. CONCLUSION: The low incidence of factor V Leiden and prothrombin gene G20210A mutation suggest that they are not the major genetic risk factors for thrombophilia in the Chinese.
OBJECTIVE: To evaluate the incidence of factor V Leiden and prothrombin gene G20210A mutation in patients with venous thrombosis and healthy volunteers. METHODS:Factor V Leiden and prothrombin gene G20210A mutation were analysed in 97 cases of venous thrombosis and 100 healthy volunteers with the methods of one-step PCR-RFLP. RESULTS: PCR products for the factor V gene (175 bp) and for the prothrombin gene (118 bp) were identified to 157 bp and 98 bp fragments by electrophoresis after Taq I treatment. No factor V Leiden and prothrombin gene G20210A mutation were found in either group. CONCLUSION: The low incidence of factor V Leiden and prothrombin gene G20210A mutation suggest that they are not the major genetic risk factors for thrombophilia in the Chinese.