OBJECTIVE AND DESIGN: Since some plant and mammalian lectins specific for monosaccharides are able to induce neutrophil migration, we studied the neutrophil migration-inducing activities of marine algal lectins, specific for complex oligosaccharides from glycoproteins, from Amansia multifida (AM), Bryothamnion seaforthii (BS), Bryothamnion triquetrum (BT) and Gracilaria caudata (GC). MATERIALS AND METHODS: The neutrophil migration-inducing activity of AM, BS, BT and GC was assayed in vitro and in vivo in the peritoneal cavity or dorsal air pouch of rats or mice, and was inhibited by glycans. RESULTS: AM, BS, BT and GC induced neutrophil migration in vivo and in vitro, determining bell-shaped dose-dependent curves. Maximal neutrophil influx was determined by BT in rats and by AM in mice. Maximal human neutrophil chemotaxis was obtained with GC. These activities were not inhibited by glycoproteins previously identified as being recognized by these lectins. D-mannose was a strong inhibitor, especially of BT activity both in vitro and in vivo. CONCLUSIONS: Algal lectins induced neutrophil migration, which was inhibited by a monosaccharide, contrasting with the view that they only recognize complex oligosaccharides. Neutrophil chemotaxis assays are appropriate to study low molecular mass lectins containing a single carbohydrate recognition domain, as is the case of some lectins from algae and mammals.
OBJECTIVE AND DESIGN: Since some plant and mammalian lectins specific for monosaccharides are able to induce neutrophil migration, we studied the neutrophil migration-inducing activities of marine algal lectins, specific for complex oligosaccharides from glycoproteins, from Amansia multifida (AM), Bryothamnion seaforthii (BS), Bryothamnion triquetrum (BT) and Gracilaria caudata (GC). MATERIALS AND METHODS: The neutrophil migration-inducing activity of AM, BS, BT and GC was assayed in vitro and in vivo in the peritoneal cavity or dorsal air pouch of rats or mice, and was inhibited by glycans. RESULTS:AM, BS, BT and GC induced neutrophil migration in vivo and in vitro, determining bell-shaped dose-dependent curves. Maximal neutrophil influx was determined by BT in rats and by AM in mice. Maximal human neutrophil chemotaxis was obtained with GC. These activities were not inhibited by glycoproteins previously identified as being recognized by these lectins. D-mannose was a strong inhibitor, especially of BT activity both in vitro and in vivo. CONCLUSIONS: Algal lectins induced neutrophil migration, which was inhibited by a monosaccharide, contrasting with the view that they only recognize complex oligosaccharides. Neutrophil chemotaxis assays are appropriate to study low molecular mass lectins containing a single carbohydrate recognition domain, as is the case of some lectins from algae and mammals.
Authors: Flávio da S Bitencourt; Jozi G Figueiredo; Mário R L Mota; Carla C R Bezerra; Priscila P Silvestre; Marcus R Vale; Kyria S Nascimento; Alexandre H Sampaio; Celso S Nagano; Silvana Saker-Sampaio; Wladimir R L Farias; Benildo S Cavada; Ana M S Assreuy; Nylane M N de Alencar Journal: Naunyn Schmiedebergs Arch Pharmacol Date: 2008-02-13 Impact factor: 3.000
Authors: Xuewen Du; Jie Zhou; Olgun Guvench; Frank O Sangiorgi; Xinming Li; Ning Zhou; Bing Xu Journal: Bioconjug Chem Date: 2014-05-18 Impact factor: 4.774
Authors: Luiz Gonzaga do Nascimento-Neto; Romulo Farias Carneiro; Suzete Roberta Da Silva; Bruno Rocha Da Silva; Francisco Vassiliepe Sousa Arruda; Victor Alves Carneiro; Kyria Santiago Do Nascimento; Silvana Saker-Sampaio; Valdemiro Amaro Da Silva; Ana Lúcia Figueiredo Porto; Benildo Sousa Cavada; Alexandre Holanda Sampaio; Edson Holanda Teixeira; Celso Shiniti Nagano Journal: Mar Drugs Date: 2012-09-04 Impact factor: 6.085