Literature DB >> 11709363

Expression of green fluorescent protein as a marker for effects of antileishmanial compounds in vitro.

S W Kamau1, F Grimm, A B Hehl.   

Abstract

Transgenic Leishmania infantum promastigotes, which constitutively express green fluorescent protein (GFP) in their cytoplasm, were used to monitor the effects of antileishmanial compounds in real time. The GFP-based assay provided a reliable measure of drug-induced inhibitory effects on protein expression, resulting in a dynamic picture of the responses of leishmanial promastigotes to the compounds tested.

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Year:  2001        PMID: 11709363      PMCID: PMC90892          DOI: 10.1128/AAC.45.12.3654-3656.2001

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN: 0066-4804            Impact factor:   5.191


  24 in total

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2.  Promastigote infectivity in Leishmania infantum.

Authors:  F Grimm; R Brun; L Jenni
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5.  Green fluorescent protein as a marker for gene expression.

Authors:  M Chalfie; Y Tu; G Euskirchen; W W Ward; D C Prasher
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Authors:  T Cavaliero; P Arnold; A Mathis; T Glaus; R Hofmann-Lehmann; P Deplazes
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7.  Use of the green fluorescent protein as a marker in transfected Leishmania.

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Journal:  Mol Biochem Parasitol       Date:  1996-04       Impact factor: 1.759

8.  Short- and long-term efficacy of hexadecylphosphocholine against established Leishmania infantum infection in BALB/c mice.

Authors:  Y Le Fichoux; D Rousseau; B Ferrua; S Ruette; A Lelièvre; D Grousson; J Kubar
Journal:  Antimicrob Agents Chemother       Date:  1998-03       Impact factor: 5.191

9.  Flow cytometric assessment of allopurinol susceptibility in Leishmania infantum promastigote.

Authors:  S W Kamau; M Hurtado; U U Müller-Doblies; F Grimm; R Nunez
Journal:  Cytometry       Date:  2000-08-01

10.  Green fluorescent protein reporter microplate assay for high-throughput screening of compounds against Mycobacterium tuberculosis.

Authors:  L A Collins; M N Torrero; S G Franzblau
Journal:  Antimicrob Agents Chemother       Date:  1998-02       Impact factor: 5.191

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  21 in total

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5.  Antileishmanial potential of a marine sponge, Haliclona exigua (Kirkpatrick) against experimental visceral leishmaniasis.

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6.  An alternative in vitro drug screening test using Leishmania amazonensis transfected with red fluorescent protein.

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7.  Real-time in vivo green fluorescent protein imaging of a murine leishmaniasis model as a new tool for Leishmania vaccine and drug discovery.

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8.  Development of a fluorescence-based assay to screen antiviral drugs against Kaposi's sarcoma associated herpesvirus.

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10.  Simple colorimetric trypanothione reductase-based assay for high-throughput screening of drugs against Leishmania intracellular amastigotes.

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Journal:  Antimicrob Agents Chemother       Date:  2013-11-04       Impact factor: 5.191

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