Literature DB >> 11709351

Chloramphenicol-sensitive Escherichia coli strain expressing the chloramphenicol acetyltransferase (cat) gene.

J Potrykus1, G Wegrzyn.   

Abstract

An Escherichia coli strain (strain CM2555) bearing the chloramphenicol acetyltransferase (cat) gene was found to be sensitive to chloramphenicol. We demonstrate that the cat gene is efficiently expressed in strain CM2555. Our results suggest that decreased levels of acetyl coenzyme A in cat-expressing CM2555 cells in the presence of chloramphenicol may cause the bacterium to be sensitive to this antibiotic.

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Year:  2001        PMID： 11709351      PMCID： PMC90880          DOI： 10.1128/AAC.45.12.3610-3612.2001

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  22 in total

1.  The effect of some antibiotic-resistance-conferring plasmids on the removal of the heat-aggregated proteins from Escherichia coli cells.

Authors:  S Kedzierska; M Staniszewska; J Potrykus; G Wegrzyn
Journal:  FEMS Microbiol Lett       Date:  1999-07-15       Impact factor: 2.742

Review 2.  Multidrug resistance mechanisms: drug efflux across two membranes.

Authors:  H I Zgurskaya; H Nikaido
Journal:  Mol Microbiol       Date:  2000-07       Impact factor: 3.501

3.  Characterization and comparison of chloramphenicol acetyltransferase variants.

Authors:  Y Zaidenzaig; J E Fitton; L C Packman; W V Shaw
Journal:  Eur J Biochem       Date:  1979-10-15

4.  Characterization of the dnaA, gyrB and other genes in the dnaA region of the Escherichia coli chromosome on specialized transducing phages lambda tna.

Authors:  F G Hansen; K von Meyenburg
Journal:  Mol Gen Genet       Date:  1979-09

5.  Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.

Authors:  A C Chang; S N Cohen
Journal:  J Bacteriol       Date:  1978-06       Impact factor: 3.490

6.  Plasmids of Escherichia coli as cloning vectors.

Authors:  F Bolivar; K Backman
Journal:  Methods Enzymol       Date:  1979       Impact factor: 1.600

7.  The enzymic interconversion of acetate and acetyl-coenzyme A in Escherichia coli.

Authors:  T D Brown; M C Jones-Mortimer; H L Kornberg
Journal:  J Gen Microbiol       Date:  1977-10

8.  Primary structure of a chloramphenicol acetyltransferase specified by R plasmids.

Authors:  W V Shaw; L C Packman; B D Burleigh; A Dell; H R Morris; B S Hartley
Journal:  Nature       Date:  1979 Dec 20-27       Impact factor: 49.962

9.  The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.

Authors:  J Vieira; J Messing
Journal:  Gene       Date:  1982-10       Impact factor: 3.688

Review 10.  Chloramphenicol acetyltransferase: enzymology and molecular biology.

Authors:  W V Shaw
Journal:  CRC Crit Rev Biochem       Date:  1983
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5.  The Genomic Basis of Intrinsic and Acquired Antibiotic Resistance in the Genus Serratia.

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