Literature DB >> 11708655

Improvement of in-rumen digestibility of alfalfa forage by genetic manipulation of lignin O-methyltransferases.

D Guo1, F Chen, J Wheeler, J Winder, S Selman, M Peterson, R A Dixon.   

Abstract

Lignin inhibits forage digestibility by ruminant animals, and lignin levels and the proportion of dimethylated syringyl (S) lignin monomers increase with progressive maturity in stems of forage crops. We generated transgenic alfalfa (Medicago sativa L.) with reduced lignin content and altered lignin composition. Down-regulation of caffeic acid 3-O-methyltransferase (COMT) reduces lignin content, accompanied by near total loss of S lignin, whereas down-regulation of caffeoyl coenzyme A 3-O-methyltransferase (CCoAOMT) reduces lignin content without reduction in S lignin. These changes are not accompanied by altered ratios of cell wall polysaccharides. Analysis of rumen digestibility of alfalfa forage in fistulated steers revealed improved digestibility of forage from COMT down-regulated plants, but a greater improvement in digestibility following down-regulation of CCoAOMT. The results indicate that both lignin content and composition affect digestibility of alfalfa forage, and reveal a new strategy for forage quality improvement by genetic manipulation of CCoAOMT expression.

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Year:  2001        PMID： 11708655     DOI： 10.1023/a:1012278106147

Source DB:  PubMed          Journal:  Transgenic Res        ISSN： 0962-8819            Impact factor:   2.788


  13 in total

1.  cis-element combinations determine phenylalanine ammonia-lyase gene tissue-specific expression patterns.

Authors:  A Leyva; X Liang; J A Pintor-Toro; R A Dixon; C J Lamb
Journal:  Plant Cell       Date:  1992-03       Impact factor: 11.277

2.  A simple method that uses differential staining and light microscopy to assess the selectivity of wood delignification by white rot fungi.

Authors:  E Srebotnik; K Messner
Journal:  Appl Environ Microbiol       Date:  1994-04       Impact factor: 4.792

3.  Substrate preferences of O-methyltransferases in alfalfa suggest new pathways for 3-O-methylation of monolignols.

Authors:  K Parvathi; F Chen; D Guo; J W Blount; R A Dixon
Journal:  Plant J       Date:  2001-01       Impact factor: 6.417

4.  Influence of lignin on digestibility of forage cell wall material.

Authors:  H G Jung; K P Vogel
Journal:  J Anim Sci       Date:  1986-06       Impact factor: 3.159

5.  Down-regulation of cinnamyl alcohol dehydrogenase in transgenic alfalfa (Medicago sativa L.) and the effect on lignin composition and digestibility.

Authors:  M Baucher; M A Bernard-Vailhé; B Chabbert; J M Besle; C Opsomer; M Van Montagu; J Botterman
Journal:  Plant Mol Biol       Date:  1999-02       Impact factor: 4.076

6.  Coniferyl aldehyde 5-hydroxylation and methylation direct syringyl lignin biosynthesis in angiosperms.

Authors:  K Osakabe; C C Tsao; L Li; J L Popko; T Umezawa; D T Carraway; R H Smeltzer; C P Joshi; V L Chiang
Journal:  Proc Natl Acad Sci U S A       Date:  1999-08-03       Impact factor: 11.205

7.  Elucidation of factors associated with the maturity-related decline in degradability of big bluestem cell wall.

Authors:  E C Titgemeyer; R C Cochran; E G Towne; C K Armendariz; K C Olson
Journal:  J Anim Sci       Date:  1996-03       Impact factor: 3.159

8.  New routes for lignin biosynthesis defined by biochemical characterization of recombinant ferulate 5-hydroxylase, a multifunctional cytochrome P450-dependent monooxygenase.

Authors:  J M Humphreys; M R Hemm; C Chapple
Journal:  Proc Natl Acad Sci U S A       Date:  1999-08-31       Impact factor: 11.205

9.  Metabolic changes in elicitor-treated bean cells. Enzymic responses associated with rapid changes in cell wall components.

Authors:  G P Bolwell; M P Robbins; R A Dixon
Journal:  Eur J Biochem       Date:  1985-05-02

10.  Developmental expression and substrate specificities of alfalfa caffeic acid 3-O-methyltransferase and caffeoyl coenzyme A 3-O-methyltransferase in relation to lignification.

Authors:  K Inoue; V J Sewalt; G B Murray; W Ni; C Stürzer; R A Dixon
Journal:  Plant Physiol       Date:  1998-07       Impact factor: 8.340
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  45 in total

1.  A polymer of caffeyl alcohol in plant seeds.

Authors:  Fang Chen; Yuki Tobimatsu; Daphna Havkin-Frenkel; Richard A Dixon; John Ralph
Journal:  Proc Natl Acad Sci U S A       Date:  2012-01-17       Impact factor: 11.205

2.  Targeted down-regulation of cytochrome P450 enzymes for forage quality improvement in alfalfa (Medicago sativa L.).

Authors:  M S Srinivasa Reddy; Fang Chen; Gail Shadle; Lisa Jackson; Hugh Aljoe; Richard A Dixon
Journal:  Proc Natl Acad Sci U S A       Date:  2005-11-01       Impact factor: 11.205

3.  Development of an in planta method for transformation of alfalfa (Medicago sativa).

Authors:  J Troy Weeks; Jingsong Ye; Caius M Rommens
Journal:  Transgenic Res       Date:  2007-09-13       Impact factor: 2.788

4.  Lignin modification leads to increased nodule numbers in alfalfa.

Authors:  Lina Gallego-Giraldo; Kishor Bhattarai; Catalina I Pislariu; Jin Nakashima; Yusuke Jikumaru; Yuji Kamiya; Michael K Udvardi; Maria J Monteros; Richard A Dixon
Journal:  Plant Physiol       Date:  2014-01-09       Impact factor: 8.340

5.  Down-regulation of caffeic acid o-methyltransferase in maize revisited using a transgenic approach.

Authors:  Joel Piquemal; Simon Chamayou; Isabelle Nadaud; Michel Beckert; Yves Barrière; Isabelle Mila; Catherine Lapierre; Joan Rigau; Pere Puigdomenech; Alain Jauneau; Catherine Digonnet; Alain-Michel Boudet; Deborah Goffner; Magalie Pichon
Journal:  Plant Physiol       Date:  2002-12       Impact factor: 8.340

Review 6.  Biotechnological advancements in alfalfa improvement.

Authors:  Suresh Kumar
Journal:  J Appl Genet       Date:  2011-01-29       Impact factor: 3.240

7.  Metabolic engineering of 2-phenylethanol pathway producing fragrance chemical and reducing lignin in Arabidopsis.

Authors:  Guang Qi; Dian Wang; Li Yu; Xianfeng Tang; Guohua Chai; Guo He; Wenxuan Ma; Shengying Li; Yingzhen Kong; Chunxiang Fu; Gongke Zhou
Journal:  Plant Cell Rep       Date:  2015-04-21       Impact factor: 4.570

8.  A genomics approach to deciphering lignin biosynthesis in switchgrass.

Authors:  Hui Shen; Mitra Mazarei; Hiroshi Hisano; Luis Escamilla-Trevino; Chunxiang Fu; Yunqiao Pu; Mary R Rudis; Yuhong Tang; Xirong Xiao; Lisa Jackson; Guifen Li; Tim Hernandez; Fang Chen; Arthur J Ragauskas; C Neal Stewart; Zeng-Yu Wang; Richard A Dixon
Journal:  Plant Cell       Date:  2013-11-27       Impact factor: 11.277

9.  QTL detection for forage quality and stem histology in four connected mapping populations of the model legume Medicago truncatula.

Authors:  Luz Del Carmen Lagunes Espinoza; Bernadette Julier
Journal:  Theor Appl Genet       Date:  2012-10-26       Impact factor: 5.699

10.  A new Arabidopsis thaliana mutant deficient in the expression of O-methyltransferase impacts lignins and sinapoyl esters.

Authors:  Thomas Goujon; Richard Sibout; Brigitte Pollet; Bruno Maba; Laurent Nussaume; Nicole Bechtold; Fachuang Lu; John Ralph; Isabelle Mila; Yves Barrière; Catherine Lapierre; Lise Jouanin
Journal:  Plant Mol Biol       Date:  2003-04       Impact factor: 4.076
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