Comparison of radioimmunoprecipitation assays for the detection of human anti-tumor virus antibodies.

The demonstration of human antibodies reactive in radioimmunoprecipitation assays (RIAs) with primate tumor virus (oncornavirus) antigen has implications for a possible previously published negative findings and led to considerable scientific controversy. We feel much of the discrepancy may be of methodological origin. An attempt is therefore made in this communication to resolve these apparent discrepancies by comparing various published parameters of the RIAs used in the search for human antibodies reactive with oncornavirus antigens.