Literature DB >> 116996

The separation of basal and differentiating cells from human epidermis for DNA cytofluorometry.

Y Sasai, K Kawamura, K Namba.   

Abstract

A method for the selective separation of human epidermal cells, in which the Feulgen-stainable material suffers minimal damage, was investigated. The principal stage involves alpha-chymotrypsin treatment of skin specimens as well as shaking in an isotonic solution. With respect to DNA distribution pattern, there was good agreement between that of epidermal cells separated with the microdissection-ultrasonic irradiation method, reported previously by us, and those separated by the present method.

Entities:  

Mesh：

Substances：

Year:  1979        PMID： 116996     DOI： 10.1007/bf00490055

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  13 in total

1.  MOUSE EPIDERMAL CELLS AND CARCINOGENESIS. I. ISOLATION OF SKIN CONSTITUENTS.

Authors:  B C GIOVANELLA; C HEIDELBERGER
Journal:  Cancer Res       Date:  1965-02       Impact factor: 12.701

2.  Nucleic acid content in mouse epidermis separated from dermis by different methods.

Authors:  R G TSANEV; G G MARKOV
Journal:  Experientia       Date:  1960-10-15

3.  Methods for the study of the cytochemistry of nucleic acids and chromosomes.

Authors:  R R COWDEN
Journal:  Mikroskopie       Date:  1957-04

4.  The role of divalent cations in epidermolysis.

Authors:  R L Dimond; K L Erickson; K D Wuepper
Journal:  Br J Dermatol       Date:  1976-07       Impact factor: 9.302

5.  Selective separation of mouse epidermal cells and cytophotometric estimation of their nuclear DNA.

Authors:  M Alavaikko
Journal:  Acta Pathol Microbiol Scand Suppl       Date:  1971

6.  Loss of basic nuclear protein during fermentative isolation of epidermal cells.

Authors:  J Filkuka; B Lederer; W Sandritter
Journal:  Acta Histochem       Date:  1968       Impact factor: 2.479

7.  Oxygen consumption of basal and differentiating cells from hairless mouse epidermis. A new method for obtaining almost pure selections of basal and differentiating cells respectively.

Authors:  O D Laerum
Journal:  J Invest Dermatol       Date:  1969-02       Impact factor: 8.551

8.  Separation of mouse epidermal basal and differentiating cells for microflow fluorometric measurements: a methodologic study.

Authors:  O P Clausen; T Lindmo; K Sandnes; E Thorud
Journal:  Virchows Arch B Cell Pathol       Date:  1976-05-26

9.  Loss of nuclear acid during fermentative treatment of human lymphocytes.

Authors:  Y Sasai; K Kawamura; K Namba
Journal:  Kurume Med J       Date:  1979

10.  Standardization of the post-irradiation method to eliminate primary fluorescence in cytofluorometry.

Authors:  M Fukuda; K Nakanishi; I Sawamura; S Fujita
Journal:  Histochemistry       Date:  1977-05-20
View more
  4 in total

1.  Nuclear differentiation and ultimate fate during epidermal keratinization. Two-wavelength and cytofluorometric DNA investigations completed by computerized scanning image analysis.

Authors:  D Broekaert; P Van Oostveldt; P Coucke; J De Bersaques; E Gillis; P Reyniers
Journal:  Arch Dermatol Res       Date:  1986       Impact factor: 3.017

2.  Nrf2 establishes a glutathione-mediated gradient of UVB cytoprotection in the epidermis.

Authors:  Matthias Schäfer; Sabine Dütsch; Ulrich auf dem Keller; Fatemeh Navid; Agatha Schwarz; Delinda A Johnson; Jeffrey A Johnson; Sabine Werner
Journal:  Genes Dev       Date:  2010-05-15       Impact factor: 11.361

3.  Fractionation of Paget cells in extramammary Paget's disease.

Authors:  O Mori; H Hachisuka; S Nakano; Y Sasai
Journal:  Arch Dermatol Res       Date:  1993       Impact factor: 3.017

4.  Separation of keratinocytes by density gradient centrifugation for DNA cytofluorometry.

Authors:  Y Sasai; H Hachisuka; O Mori; H Nomura
Journal:  Histochemistry       Date:  1984
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.