| Literature DB >> 11696850 |
E R Argañaraz1, G B Hubbard, L A Ramos, A L Ford, N Nitz, M M Leland, J L Vandeberg, A R Teixeira.
Abstract
Trypanosoma cruzi (Schyzotrypanum, Chagas, 1909), and Chagas disease are endemic in captive-reared baboons at the Southwest Foundation for Biomedical Research, San Antonio, Texas. We obtained PCR amplification products from DNA extracted from sucking lice collected from the hair and skin of T. cruzi-infected baboons, with specific nested sets of primers for the protozoan kinetoplast DNA, and nuclear DNA. These products were hybridized to their complementary internal sequences. Selected sequences were cloned and sequencing established the presence of T. cruzi nuclear DNA, and minicircle kDNA. Competitive PCR with a kDNA set of primers determined the quantity of approximately 23.9 +/- 18.2 T. cruzi per louse. This finding suggests that the louse may be a vector incidentally contributing to the dissemination of T. cruzi infection in the baboon colony.Entities:
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Year: 2001 PMID： 11696850 DOI： 10.1590/s0036-46652001000500007
Source DB: PubMed Journal: Rev Inst Med Trop Sao Paulo ISSN： 0036-4665 Impact factor: 1.846