| Literature DB >> 11694305 |
H Gadgil1, S A Oak, H W Jarrett.
Abstract
The focus of this review is on DNA affinity chromatography, which is the most powerful tool for purification of DNA binding proteins. The use of nonspecific-, sequence specific- and single stranded-DNA affinity columns in purification of various DNA binding proteins is discussed. The purification strategies for transcription factors, restriction enzymes, telomerases, DNA and RNA polymerase and DNA binding antibodies are described. Different applications of DNA affinity chromatography are presented.Mesh：
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Year: 2001 PMID： 11694305 DOI： 10.1016/s0165-022x(01)00223-8
Source DB: PubMed Journal: J Biochem Biophys Methods ISSN： 0165-022X