AIMS/HYPOTHESIS: The NPHS1 gene product, nephrin, is a crucial component of the glomerular filtration barrier preventing proteinuria and previously assumed to be kidney-specific. The aim of this study was to describe the expression of nephrin mRNA and protein in human pancreas as well as identify the nephrin-expressing cell types. METHODS: RNA dot blot, reverse transcriptase-polymerase chain reaction, sequencing, immunoblotting and dual immunofluorescence were used for the characterisation of nephrin in the pancreas. RESULTS: Except for the kidney, the pancreas was found to be the only tissue expressing nephrin as screened with a human tissue RNA dot blot. The expression was verified with reverse transcriptase-polymerase chain reaction and by sequencing nephrin from a human pancreatic complementary DNA library. Nephrin antibody in immunoblot detected a 165,000 M(r) protein in the pancreas. Dual immunofluorescence showed that nephrin was specifically localised in the beta cells of the islets of Langerhans. There was no overlap with glucagon, somatostatin, or the ductal cell marker cytokeratin 19. CONCLUSION/ INTERPRETATION: These data show that nephrin is a novel molecule of pancreatic beta cells.
AIMS/HYPOTHESIS: The NPHS1 gene product, nephrin, is a crucial component of the glomerular filtration barrier preventing proteinuria and previously assumed to be kidney-specific. The aim of this study was to describe the expression of nephrin mRNA and protein in humanpancreas as well as identify the nephrin-expressing cell types. METHODS: RNA dot blot, reverse transcriptase-polymerase chain reaction, sequencing, immunoblotting and dual immunofluorescence were used for the characterisation of nephrin in the pancreas. RESULTS: Except for the kidney, the pancreas was found to be the only tissue expressing nephrin as screened with a human tissue RNA dot blot. The expression was verified with reverse transcriptase-polymerase chain reaction and by sequencing nephrin from a humanpancreatic complementary DNA library. Nephrin antibody in immunoblot detected a 165,000 M(r) protein in the pancreas. Dual immunofluorescence showed that nephrin was specifically localised in the beta cells of the islets of Langerhans. There was no overlap with glucagon, somatostatin, or the ductal cell marker cytokeratin 19. CONCLUSION/ INTERPRETATION: These data show that nephrin is a novel molecule of pancreatic beta cells.
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Authors: Leslie A Bruggeman; Zhenzhen Wu; Liping Luo; Sethu M Madhavan; Martha Konieczkowski; Paul E Drawz; David B Thomas; Laura Barisoni; John R Sedor; John F O'Toole Journal: J Am Soc Nephrol Date: 2016-03-29 Impact factor: 10.121
Authors: Alessia Fornoni; Jongmin Jeon; Javier Varona Santos; Lorenzo Cobianchi; Alexandra Jauregui; Luca Inverardi; Slavena A Mandic; Christina Bark; Kevin Johnson; George McNamara; Antonello Pileggi; R Damaris Molano; Jochen Reiser; Karl Tryggvason; Dontscho Kerjaschki; Per-Olof Berggren; Peter Mundel; Camillo Ricordi Journal: Diabetes Date: 2009-10-15 Impact factor: 9.461