BACKGROUND/AIMS: Recent observations suggest that natural killer (NK) cell activity might be impaired in chronic hepatitis C. However, to date antibody-dependent cellular cytotoxicity (ADCC) has not been studied in chronic hepatitis C in detail. METHODS: Therefore, we investigated spontaneous and cytokine-induced (interleukin-2 and interferon-gamma) natural cytotoxicity and ADCC in 29 patients suffering from chronic hepatitis C and 19 healthy controls. Cytotoxicity was determined with a flow-cytometric assay, which can also assess monocyte cytotoxicity. As target cells we used the colorectal tumor cell line HT29 and the lymphoma cell line Raji. RESULTS: We found no significant differences with respect to spontaneous cytotoxicity (HCV versus healthy controls (32 vs. 46%) and 17-1A specific ADCC (59 vs. 48%), even if isolated monocytes or NK cells were studied. Preincubation and stimulation of effector cells with cytokines increased both natural cytotoxicity and ADCC by 20-30%. However, natural cytotoxicity and ADCC after stimulation did not differ between the two groups. CONCLUSIONS: Our data obtained with a long-term cytotoxicity assay do not reveal impaired cytolytic capacity of the innate immune system in chronic hepatitis C, even when isolated monocytes and NK cells were studied as effector cells.
BACKGROUND/AIMS: Recent observations suggest that natural killer (NK) cell activity might be impaired in chronic hepatitis C. However, to date antibody-dependent cellular cytotoxicity (ADCC) has not been studied in chronic hepatitis C in detail. METHODS: Therefore, we investigated spontaneous and cytokine-induced (interleukin-2 and interferon-gamma) natural cytotoxicity and ADCC in 29 patients suffering from chronic hepatitis C and 19 healthy controls. Cytotoxicity was determined with a flow-cytometric assay, which can also assess monocyte cytotoxicity. As target cells we used the colorectal tumor cell line HT29 and the lymphoma cell line Raji. RESULTS: We found no significant differences with respect to spontaneous cytotoxicity (HCV versus healthy controls (32 vs. 46%) and 17-1A specific ADCC (59 vs. 48%), even if isolated monocytes or NK cells were studied. Preincubation and stimulation of effector cells with cytokines increased both natural cytotoxicity and ADCC by 20-30%. However, natural cytotoxicity and ADCC after stimulation did not differ between the two groups. CONCLUSIONS: Our data obtained with a long-term cytotoxicity assay do not reveal impaired cytolytic capacity of the innate immune system in chronic hepatitis C, even when isolated monocytes and NK cells were studied as effector cells.
Authors: Galit Alter; Stephanie Jost; Suzannah Rihn; Laura L Reyor; Brian E Nolan; Musie Ghebremichael; Ronald Bosch; Marcus Altfeld; Georg M Lauer Journal: J Hepatol Date: 2010-12-17 Impact factor: 25.083
Authors: Ute-Christiane Meier; Rachel E Owen; Elizabeth Taylor; Andrew Worth; Nikolai Naoumov; Christian Willberg; Kwok Tang; Phillipa Newton; Pierre Pellegrino; Ian Williams; Paul Klenerman; Persephone Borrow Journal: J Virol Date: 2005-10 Impact factor: 5.103
Authors: Sara J Conry; Kimberly A Milkovich; Nicole L Yonkers; Benigno Rodriguez; Helene B Bernstein; Robert Asaad; Frederick P Heinzel; Magdalena Tary-Lehmann; Michael M Lederman; Donald D Anthony Journal: J Virol Date: 2009-08-19 Impact factor: 5.103
Authors: Nicole L Yonkers; Kimberly A Milkovich; Benigno Rodriguez; Anthony B Post; Robert Asaad; Frederick P Heinzel; Hernan Valdez; Magdalena Tary-Lehmann; Donald D Anthony Journal: Clin Immunol Date: 2009-02-03 Impact factor: 3.969