The effect of the choline head group on phospholipid hydration.

The hydration behavior of three naturally occurring sphingomyelins (SM) has been studied. Adsorption isotherms for these SM, singly and in combination with other lipids, were obtained and the isotherms analyzed by the application of BET theory. The results are compared with those found previously by us for the phosphatidylcholines (PC). We find that, depending on the SM studied, both "weak" and "strong" water adsorption can be observed and thus, the presence of choline in the phospholipid head group, does not guarantee "strong" water adsorption. When the head group is removed from SM, the resulting ceramides are found to be very weak, water adsorbers. Cholesterol, when present in a mixture with SM, has a rather dramatic effect on the hydration of the SM. A mixed system of PC and SM exhibits water adsorption characteristics very similar to those exhibited by PC itself. We speculate that these hydration results may well play a role in the cell signaling action of SM and, moreover, may be closely associated with the hypothesized formation of lipid "rafts."