Literature DB >> 11684132

A sensitive and reproducible method for the detection of oligoclonal IgM bands.

L M Villar1, P González-Porqué, J Masjuán, J C Alvarez-Cermeño, A Bootello, G Keir.   

Abstract

We have developed a sensitive and specific isoelectrofocusing (IEF) method for the detection of oligoclonal (OGC) IgM in the cerebrospinal fluid (CSF) and sera. In this procedure, ampholytes, in the range pH 5-8, were used to improve the resolution, and serum was diluted in saline to avoid IgM precipitation. Samples were treated with 50 mM dithiothreitol (DTT) at pH 9.5 to reduce IgM and improve the migration of the protein. Using an anti-IgM labelled with alkaline phosphatase, the limit of detection was found to be 0.1 ng in 5 microl of sample.

Entities:  

Mesh：

Substances：

Year:  2001        PMID： 11684132     DOI： 10.1016/s0022-1759(01)00492-6

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  10 in total

1.  Cerebrospinal fluid anti-Epstein-Barr virus specific oligoclonal IgM and IgG bands in patients with clinically isolated and Guillain-Barré syndrome.

Authors:  Diana Ferraro; Veronica Galli; Anna Maria Simone; Roberta Bedin; Francesca Vitetta; Elisa Merelli; Paolo Frigio Nichelli; Patrizia Sola
Journal:  J Neurovirol       Date:  2016-11-22       Impact factor: 2.643

2.  Intrathecal synthesis of oligoclonal IgM against myelin lipids predicts an aggressive disease course in MS.

Authors:  Luisa M Villar; María C Sádaba; Ernesto Roldán; Jaime Masjuan; Pedro González-Porqué; Noelia Villarrubia; Mercedes Espiño; José A García-Trujillo; Alfredo Bootello; José C Alvarez-Cermeño
Journal:  J Clin Invest       Date:  2005-01       Impact factor: 14.808

3.  IL-2, IL-6 and chitinase 3-like 2 might predict early relapse activity in multiple sclerosis.

Authors:  Marko Petržalka; Eva Meluzínová; Jana Libertínová; Hana Mojžišová; Jitka Hanzalová; Petra Ročková; Martin Elišák; Silvia Kmetonyová; Jan Šanda; Ondřej Sobek; Petr Marusič
Journal:  PLoS One       Date:  2022-06-27       Impact factor: 3.752

4.  A multifactorial prognostic index in multiple sclerosis. Cerebrospinal fluid IgM oligoclonal bands and clinical features to predict the evolution of the disease.

Authors:  Jessica Mandrioli; Patrizia Sola; Roberta Bedin; Mariaelena Gambini; Elisa Merelli
Journal:  J Neurol       Date:  2008-06-13       Impact factor: 4.849

5.  Oligoclonal immunoglobulins in cerebrospinal fluid during varicella zoster virus (VZV) vasculopathy are directed against VZV.

Authors:  Mark P Burgoon; Barbara N Hammack; Gregory P Owens; Amy L Maybach; M Judith Eikelenboom; Donald H Gilden
Journal:  Ann Neurol       Date:  2003-10       Impact factor: 10.422

6.  Whole-Transcriptome Analysis in Peripheral Blood Mononuclear Cells from Patients with Lipid-Specific Oligoclonal IgM Band Characterization Reveals Two Circular RNAs and Two Linear RNAs as Biomarkers of Highly Active Disease.

Authors:  Leire Iparraguirre; Danel Olaverri; Telmo Blasco; Lucía Sepúlveda; Tamara Castillo-Triviño; Mercedes Espiño; Lucienne Costa-Frossard; Álvaro Prada; Luisa María Villar; David Otaegui; Maider Muñoz-Culla
Journal:  Biomedicines       Date:  2020-11-26

7.  Predicting Aggressive Multiple Sclerosis With Intrathecal IgM Synthesis Among Patients With a Clinically Isolated Syndrome.

Authors:  Enric Monreal; Susana Sainz de la Maza; Lucienne Costa-Frossard; Paulette Walo-Delgado; Javier Zamora; José Ignacio Fernández-Velasco; Noelia Villarrubia; Mercedes Espiño; Daniel Lourido; Paloma Lapuente; Inmaculada Toboso; José Carlos Álvarez-Cermeño; Jaime Masjuan; Luisa María Villar
Journal:  Neurol Neuroimmunol Neuroinflamm       Date:  2021-07-22

8.  Bypassing hazard of housekeeping genes: their evaluation in rat granule neurons treated with cerebrospinal fluid of multiple sclerosis subjects.

Authors:  Deepali Mathur; Juan R Urena-Peralta; Gerardo Lopez-Rodas; Bonaventura Casanova; Francisco Coret-Ferrer; Maria Burgal-Marti
Journal:  Front Cell Neurosci       Date:  2015-09-23       Impact factor: 5.505

9.  High Levels of Cerebrospinal Fluid Kappa Free Light Chains Relate to IgM Intrathecal Synthesis and Might Have Prognostic Implications in Relapsing Multiple Sclerosis.

Authors:  Jéssica Castillo-Villalba; Sara Gil-Perotín; Raquel Gasque-Rubio; Laura Cubas-Nuñez; Sara Carratalà-Boscà; Carmen Alcalá; Carlos Quintanilla-Bordás; Francisco Pérez-Miralles; Cristina Ferrer; Antonio Cañada Martínez; Jordi Tortosa; Luís Solís-Tarazona; Luisa Campos; Alberto Leivas; Begoña Laíz Marro; Bonaventura Casanova
Journal:  Front Immunol       Date:  2022-03-07       Impact factor: 7.561

10.  High prevalence of intrathecal IgA synthesis in multiple sclerosis patients.

Authors:  Úrsula Muñoz; Cristina Sebal; Esther Escudero; Maria Isabel García Sánchez; Elena Urcelay; Asier Jayo; Rafael Arroyo; Maria A García-Martínez; Roberto Álvarez-Lafuente; María C Sádaba
Journal:  Sci Rep       Date:  2022-03-11       Impact factor: 4.379
  10 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.