Improved MALDI-MS analysis of oligonucleotides through the use of fucose as a matrix additive.

With the development of matrix additives, the MALDI-MS analysis of oligonucleotides has improved greatly. When the monosaccharide fucose is combined with the matrix, the homogeneity of the MALDI target, signal strength, and signal duration are increased. The sensitivity of the MALDI experiment increases, allowing for improved detection of components in a complex mixture of oligonucleotides, such as that encountered in sequencing experiments. The addition of fucose to the matrix also causes a reduction in the extent of fragmentation of oligonucleotides.