Literature DB >> 11679365

Poly(3-hydroxybutyrate) synthesis genes in Azotobacter sp. strain FA8.

M J Pettinari1, G J Vázquez, D Silberschmidt, B Rehm, A Steinbüchel, B S Méndez.   

Abstract

Genes responsible for the synthesis of poly(3-hydroxybutyrate) (PHB) in Azotobacter sp. FA8 were cloned and analyzed. A PHB polymerase gene (phbC) was found downstream from genes coding for beta-ketothiolase (phbA) and acetoacetyl-coenzyme A reductase (phbB). A PHB synthase mutant was obtained by gene inactivation and used for genetic studies. The phbC gene from this strain was introduced into Ralstonia eutropha PHB-4 (phbC-negative mutant), and the recombinant accumulated PHB when either glucose or octanoate was used as a source of carbon, indicating that this PHB synthase cannot incorporate medium-chain-length hydroxyalkanoates into PHB.

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Year:  2001        PMID： 11679365      PMCID： PMC93310          DOI： 10.1128/AEM.67.11.5331-5334.2001

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


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  16 in total

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Journal:  J Bacteriol       Date:  2009-05-08       Impact factor: 3.490

7.  Effects of granule-associated protein PhaP on glycerol-dependent growth and polymer production in poly(3-hydroxybutyrate)-producing Escherichia coli.

Authors:  Alejandra de Almeida; Pablo I Nikel; Andrea M Giordano; M Julia Pettinari
Journal:  Appl Environ Microbiol       Date:  2007-10-26       Impact factor: 4.792

8.  Expression of the Azotobacter vinelandii poly-beta-hydroxybutyrate biosynthetic phbBAC operon is driven by two overlapping promoters and is dependent on the transcriptional activator PhbR.

Authors:  Martín Peralta-Gil; Daniel Segura; Josefina Guzmán; Luis Servín-González; Guadalupe Espín
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9.  Molecular and bioengineering strategies to improve alginate and polydydroxyalkanoate production by Azotobacter vinelandii.

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10.  Complete PHB mobilization in Escherichia coli enhances the stress tolerance: a potential biotechnological application.

Authors:  Qian Wang; Hongmin Yu; Yongzhen Xia; Zhen Kang; Qingsheng Qi
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