Literature DB >> 11668672

Efficient FLP recombination in mouse ES cells and oocytes.

J Schaft1, R Ashery-Padan, F van der Hoeven, P Gruss, A F Stewart.   

Abstract

We report an improved vector, pCAGGS-FLPe, for transient expression of the enhanced FLP recombinase in mouse ES cells and oocytes. In standard transfection experiments, about 6% of total ES colonies showed FLP recombination, albeit with mosaicism within each colony. After microinjection of pCAGGS-FLPe into oocytes, about one-third of heterozygotic mice born showed complete FLP recombination. Thus pCAGGS-FLPe presents two practical options for removal of FRT cassettes in mice. Copyright 2001 Wiley-Liss, Inc.

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Year:  2001        PMID： 11668672     DOI： 10.1002/gene.1076

Source DB:  PubMed          Journal:  Genesis        ISSN： 1526-954X            Impact factor:   2.487


  59 in total

1.  Stable and efficient cassette exchange under non-selectable conditions by combined use of two site-specific recombinases.

Authors:  Matthias Lauth; Fabio Spreafico; Kathrin Dethleffsen; Michael Meyer
Journal:  Nucleic Acids Res       Date:  2002-11-01       Impact factor: 16.971

2.  The region 3' to Xist mediates X chromosome counting and H3 Lys-4 dimethylation within the Xist gene.

Authors:  Céline Morey; Pablo Navarro; Emmanuel Debrand; Philip Avner; Claire Rougeulle; Philippe Clerc
Journal:  EMBO J       Date:  2004-01-29       Impact factor: 11.598

Review 3.  Conditional gene expression in the mouse inner ear using Cre-loxP.

Authors:  Brandon C Cox; Zhiyong Liu; Marcia M Mellado Lagarde; Jian Zuo
Journal:  J Assoc Res Otolaryngol       Date:  2012-04-24

Review 4.  Molecular neuroanatomy's "Three Gs": a primer.

Authors:  Susan M Dymecki; Jun Chul Kim
Journal:  Neuron       Date:  2007-04-05       Impact factor: 17.173

5.  Structure-function Studies in Mouse Embryonic Stem Cells Using Recombinase-mediated Cassette Exchange.

Authors:  Tim Pieters; Lieven Haenebalcke; Kenneth Bruneel; Niels Vandamme; Tino Hochepied; Jolanda van Hengel; Dagmar Wirth; Geert Berx; Jody J Haigh; Frans van Roy; Steven Goossens
Journal:  J Vis Exp       Date:  2017-04-27       Impact factor: 1.355

6.  A piggyBac transposon-based genome-wide library of insertionally mutated Blm-deficient murine ES cells.

Authors:  Wei Wang; Allan Bradley; Yue Huang
Journal:  Genome Res       Date:  2009-02-20       Impact factor: 9.043

7.  Essential role for mitochondrial thioredoxin reductase in hematopoiesis, heart development, and heart function.

Authors:  Marcus Conrad; Cemile Jakupoglu; Stéphanie G Moreno; Stefanie Lippl; Ana Banjac; Manuela Schneider; Heike Beck; Antonis K Hatzopoulos; Ursula Just; Fred Sinowatz; Wolfgang Schmahl; Kenneth R Chien; Wolfgang Wurst; Georg W Bornkamm; Markus Brielmeier
Journal:  Mol Cell Biol       Date:  2004-11       Impact factor: 4.272

8.  A novel mouse model reveals that polycystin-1 deficiency in ependyma and choroid plexus results in dysfunctional cilia and hydrocephalus.

Authors:  Claas Wodarczyk; Isaline Rowe; Marco Chiaravalli; Monika Pema; Feng Qian; Alessandra Boletta
Journal:  PLoS One       Date:  2009-09-23       Impact factor: 3.240

9.  Inducible expression of coding and inhibitory RNAs from retargetable genomic loci.

Authors:  Ina Weidenfeld; Manfred Gossen; Rainer Löw; David Kentner; Stefan Berger; Dirk Görlich; Dusan Bartsch; Hermann Bujard; Kai Schönig
Journal:  Nucleic Acids Res       Date:  2009-03-05       Impact factor: 16.971

10.  High-efficient FLPo deleter mice in C57BL/6J background.

Authors:  Yingjie Wu; Chunxin Wang; Hui Sun; Derek LeRoith; Shoshana Yakar
Journal:  PLoS One       Date:  2009-11-26       Impact factor: 3.240
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