Identification of a novel truncated isoform of trkB in the kitten primary visual cortex.

Neurotrophins have been shown to play important roles in development and plasticity of the visual cortex (VC). Since signal transduction of neurotrophins is mediated through neurotrophin receptors, we attempted to analyze neurotrophin receptors in the VC. In this study, we isolated cDNAs encoding the intracellular regions of truncated isoforms of the trkB receptor from 30-d-old kitten primary VC. Two distinct truncated isoforms of trkB were isolated and characterized by sequence analyses. One of the isoforms corresponds to the previously described truncated trkB in several mammalian species. The second isoform represents a novel truncated trkB variant form in the kitten VC. Sequence analysis revealed that this contains a sequence that has not yet been reported in any species. This novel isoform, designated trkB.T4, results from alternative splicing 189-bp (63 amino acids) downstream from the splice site giving rise to the first known truncated isoforms of trkB. In the context of recent hypotheses regarding the function of truncated trkB receptors, sequence analysis indicates that trkB.T4 may bear putative signaling/internalization sequences.