Literature DB >> 11605970

The influence of electrostatic interactions on the detection of heme-globin complexes in ESI-MS.

A Schmidt1, M Karas.   

Abstract

The heme-globin complexes of hemoglobin and myoglobin are investigated in positive-ion mode and negative-ion mode using a nano-ESI source coupled to a quadrupole ion trap MS and an orthogonal time-of-flight MS. The extent of dissociation of these noncovalent complexes upon collisional activation and thus their gas-phase stability is strongly dependent on the polarity of the ESI-MS experiment as well as on the charge of the prosthetic group (ferri-heme [Fe3+-heme]+ vs. ferro-heme [Fe2+-heme]+/-0). The results clearly point to the important role of electrostatic interactions on the gas phase stability of noncovalent complexes and therefore the ion signals observed in ESI-MS experiments.

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Year:  2001        PMID： 11605970     DOI： 10.1016/S1044-0305(01)00294-X

Source DB:  PubMed          Journal:  J Am Soc Mass Spectrom        ISSN： 1044-0305            Impact factor:   3.109


  14 in total

1.  Effects of pH on the kinetic reaction mechanism of myoglobin unfolding studied by time-resolved electrospray ionization mass spectrometry.

Authors:  O O Sogbein; D A Simmons; L Konermann
Journal:  J Am Soc Mass Spectrom       Date:  2000-04       Impact factor: 3.109

2.  Studies on heme binding in myoglobin, hemoglobin, and cytochrome c by ion spray mass spectrometry.

Authors:  Y T Li; Y L Hsieh; J D Henion; B Ganem
Journal:  J Am Soc Mass Spectrom       Date:  1993-08       Impact factor: 3.109

3.  Bio-affinity characterization mass spectrometry.

Authors:  J E Bruce; G A Anderson; R Chen; X Cheng; D C Gale; S A Hofstadler; B L Schwartz; R D Smith
Journal:  Rapid Commun Mass Spectrom       Date:  1995       Impact factor: 2.419

Review 4.  Studying noncovalent protein complexes by electrospray ionization mass spectrometry.

Authors:  J A Loo
Journal:  Mass Spectrom Rev       Date:  1997 Jan-Feb       Impact factor: 10.946

5.  Dissociation of heme from myoglobin and cytochrome b5: comparison of behavior in solution and the gas phase.

Authors:  C L Hunter; A G Mauk; D J Douglas
Journal:  Biochemistry       Date:  1997-02-04       Impact factor: 3.162

6.  The determination of protein, oligonucleotide and peptide molecular weights by ion-spray mass spectrometry.

Authors:  T R Covey; R F Bonner; B I Shushan; J Henion
Journal:  Rapid Commun Mass Spectrom       Date:  1988-11       Impact factor: 2.419

7.  Stability of the heme-globin linkage in alphabeta dimers and isolated chains of human hemoglobin. A study of the heme transfer reaction from the immobilized proteins to albumin.

Authors:  M Gattoni; A Boffi; P Sarti; E Chiancone
Journal:  J Biol Chem       Date:  1996-04-26       Impact factor: 5.157

8.  Reconstitution of acid-denatured holomyoglobin studied by time-resolved electrospray ionization mass spectrometry.

Authors:  V W Lee; Y L Chen; L Konermann
Journal:  Anal Chem       Date:  1999-10-01       Impact factor: 6.986

9.  Dissociation of heme-globin complexes by blackbody infrared radiative dissociation: molecular specificity in the gas phase?

Authors:  D S Gross; Y Zhao; E R Williams
Journal:  J Am Soc Mass Spectrom       Date:  1997-05       Impact factor: 3.109

10.  Conformational stability of heme proteins in vacuo.

Authors:  Y Konishi; R Feng
Journal:  Biochemistry       Date:  1994-08-16       Impact factor: 3.162
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  11 in total

1.  Diffusion measurements by electrospray mass spectrometry for studying solution-phase noncovalent interactions.

Authors:  Sonya M Clark; Lars Konermann
Journal:  J Am Soc Mass Spectrom       Date:  2003-05       Impact factor: 3.109

2.  Super-atmospheric pressure electrospray ion source: applied to aqueous solution.

Authors:  Lee Chuin Chen; Mridul Kanti Mandal; Kenzo Hiraoka
Journal:  J Am Soc Mass Spectrom       Date:  2011-10-12       Impact factor: 3.109

3.  Heme binding in gas-phase holo-myoglobin cations: distal becomes proximal?

Authors:  Atim A Enyenihi; Hongqian Yang; A Jimmy Ytterberg; Yaroslav Lyutvinskiy; Roman A Zubarev
Journal:  J Am Soc Mass Spectrom       Date:  2011-07-19       Impact factor: 3.109

4.  Electrospray ionization mass spectrometry studies of noncovalent myosin VI complexes reveal a new specific calmodulin binding site.

Authors:  Guillaume Chevreux; Noelle Potier; Alain Van Dorsselaer; Amel Bahloul; Anne Houdusse; Amber Wells; H Lee Sweeney
Journal:  J Am Soc Mass Spectrom       Date:  2005-08       Impact factor: 3.109

5.  Analysis of protein mixtures by electrospray mass spectrometry: effects of conformation and desolvation behavior on the signal intensities of hemoglobin subunits.

Authors:  Mark C Kuprowski; Brian L Boys; Lars Konermann
Journal:  J Am Soc Mass Spectrom       Date:  2007-04-15       Impact factor: 3.109

6.  Selective detection of specific protein-ligand complexes by electrosonic spray-precursor ion scan tandem mass spectrometry.

Authors:  Noemi Czuczy; Maria Katona; Zoltan Takats
Journal:  J Am Soc Mass Spectrom       Date:  2008-09-18       Impact factor: 3.109

7.  Direct ionization of large proteins and protein complexes by desorption electrospray ionization-mass spectrometry.

Authors:  Carly N Ferguson; Sabrina A Benchaar; Zhixin Miao; Joseph A Loo; Hao Chen
Journal:  Anal Chem       Date:  2011-07-28       Impact factor: 6.986

8.  Characterization of acid-induced protein conformational changes and noncovalent complexes in solution by using coldspray ionization mass spectrometry.

Authors:  Na Guo; Ruiping Zhang; Fei Song; Jiuming He; Bin Xia; Zeper Abliz
Journal:  J Am Soc Mass Spectrom       Date:  2009-01-09       Impact factor: 3.109

9.  Collisional cooling enhances the ability to observe non-covalent interactions within the inducible nitric oxide synthase oxygenase domain: dimerization, complexation, and dissociation.

Authors:  Jeffrey C Smith; K W Michael Siu; Steven P Rafferty
Journal:  J Am Soc Mass Spectrom       Date:  2004-05       Impact factor: 3.109

10.  Comparative analysis of oxy-hemoglobin and aquomet-hemoglobin by hydrogen/deuterium exchange mass spectrometry.

Authors:  Modupeola A Sowole; Lars Konermann
Journal:  J Am Soc Mass Spectrom       Date:  2013-05-11       Impact factor: 3.109
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