OBJECTIVE: The use of fibrin gel, which can be produced from patients' blood, was investigated as an autologous, biodegradable scaffold. A new moulding technique was developed to create a complete aortic root. METHODS: A new moulding technique was generated for the creation of complete valve conduit. On the basis of biomechanical valve design studies, a tricuspid "ventricular" and "aortic" stamp were developed. A silicone-coated aluminum cylinder was used to circumferentially limit the mould. The cell/gel suspension was filled into the mould and polymerization was started. RESULTS: The creation of complex structures such as complete valve conduits is possible with the moulding technique described. With a layer thickness of up to 2 mm, histological investigations showed excellent tissue development with viable fibroblasts surrounded by collagen bundles. CONCLUSION: Fibrin gel unifies many properties of an ideal scaffold: The formation of complex structures is possible, the degradation and polymerization is controllable and the formation of the extracellular matrix is excellent.
OBJECTIVE: The use of fibrin gel, which can be produced from patients' blood, was investigated as an autologous, biodegradable scaffold. A new moulding technique was developed to create a complete aortic root. METHODS: A new moulding technique was generated for the creation of complete valve conduit. On the basis of biomechanical valve design studies, a tricuspid "ventricular" and "aortic" stamp were developed. A silicone-coated aluminum cylinder was used to circumferentially limit the mould. The cell/gel suspension was filled into the mould and polymerization was started. RESULTS: The creation of complex structures such as complete valve conduits is possible with the moulding technique described. With a layer thickness of up to 2 mm, histological investigations showed excellent tissue development with viable fibroblasts surrounded by collagen bundles. CONCLUSION: Fibrin gel unifies many properties of an ideal scaffold: The formation of complex structures is possible, the degradation and polymerization is controllable and the formation of the extracellular matrix is excellent.
Authors: Miriam Weber; Eriona Heta; Ricardo Moreira; Valentine N Gesche; Thomas Schermer; Julia Frese; Stefan Jockenhoevel; Petra Mela Journal: Tissue Eng Part C Methods Date: 2013-10-19 Impact factor: 3.056
Authors: Stefan Weinandy; Simone Laffar; Ronald E Unger; Thomas C Flanagan; Robert Loesel; C James Kirkpatrick; Marc van Zandvoort; Benita Hermanns-Sachweh; Agnieszka Dreier; Doris Klee; Stefan Jockenhoevel Journal: Tissue Eng Part A Date: 2014-03-03 Impact factor: 3.845
Authors: Ricardo Moreira; Valentine N Gesche; Luis G Hurtado-Aguilar; Thomas Schmitz-Rode; Julia Frese; Stefan Jockenhoevel; Petra Mela Journal: Tissue Eng Part C Methods Date: 2014-03-25 Impact factor: 3.056
Authors: Ricardo Moreira; Thaddaeus Velz; Nuno Alves; Valentine N Gesche; Axel Malischewski; Thomas Schmitz-Rode; Julia Frese; Stefan Jockenhoevel; Petra Mela Journal: Tissue Eng Part C Methods Date: 2014-12-19 Impact factor: 3.056