Literature DB >> 11604320

Gene expression monitoring in soils by mRNA analysis and gene lux fusions.

G S Sayler1, J T Fleming, D E Nivens.   

Abstract

Two methods recently developed to monitor the gene expression of microbial communities in soil are the extraction and detection of messenger RNA from soil microorganisms and the construction and use of lux-based bioreporter strains. The goal of these approaches is to assess microbial activity in natural and impacted soil environments.

Keywords:  Non-programmatic

Mesh:

Substances:

Year:  2001        PMID: 11604320     DOI: 10.1016/s0958-1669(00)00245-7

Source DB:  PubMed          Journal:  Curr Opin Biotechnol        ISSN: 0958-1669            Impact factor:   9.740


  5 in total

1.  Molecular insight into activated sludge producing polyhydroxyalkanoates under aerobic-anaerobic conditions.

Authors:  Slawomir Ciesielski; Tomasz Pokoj; Ewa Klimiuk
Journal:  J Ind Microbiol Biotechnol       Date:  2008-04-17       Impact factor: 3.346

2.  Effect of soil clay content on RNA isolation and on detection and quantification of bacterial gene transcripts in soil by quantitative reverse transcription-PCR.

Authors:  A Novinscak; M Filion
Journal:  Appl Environ Microbiol       Date:  2011-07-01       Impact factor: 4.792

3.  Effect of temperature, pH, and initial cell number on luxCDABE and nah gene expression during naphthalene and salicylate catabolism in the bioreporter organism Pseudomonas putida RB1353.

Authors:  Jonathan G Dorn; Robert J Frye; Raina M Maier
Journal:  Appl Environ Microbiol       Date:  2003-04       Impact factor: 4.792

4.  mRNA extraction and reverse transcription-PCR protocol for detection of nifH gene expression by Azotobacter vinelandii in soil.

Authors:  Helmut Bürgmann; Franco Widmer; William V Sigler; Josef Zeyer
Journal:  Appl Environ Microbiol       Date:  2003-04       Impact factor: 4.792

Review 5.  Pseudomonas fluorescens HK44: lessons learned from a model whole-cell bioreporter with a broad application history.

Authors:  Josef Trögl; Archana Chauhan; Steven Ripp; Alice C Layton; Gabriela Kuncová; Gary S Sayler
Journal:  Sensors (Basel)       Date:  2012-02-06       Impact factor: 3.576

  5 in total

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