| Literature DB >> 11602248 |
A Haouz1, J M Glandières, B Alpert.
Abstract
Dynamics of glucose oxidase immobilized and in solution were compared through their tryptophan fluorescence spectra, decay times and quenching by acrylamide. Energy barrier for thermal inactivation and melting temperature of both soluble and immobilized enzyme were also measured. Data show that the fluctuation amplitude is at the origin of protein instability.Entities:
Mesh：
Substances：
Year: 2001 PMID： 11602248 DOI： 10.1016/s0014-5793(01)02917-9
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124