| Literature DB >> 11598842 |
D R Brown1, J T Bryan, J M Schroeder, T S Robinson, K H Fife, C M Wheeler, E Barr, P R Smith, L Chiacchierini, A DiCello, K U Jansen.
Abstract
Neutralization of human papillomavirus type 11 (HPV-11) has been demonstrated using serum and cervical secretions from primates vaccinated with virus-like particles (VLPs). Theoretically, neutralizing antibodies could protect women from HPV infection. The immunogenicity of a yeast-derived HPV-11 L1 VLP vaccine was tested in women. Serum specimens were evaluated for HPV-11 titer by competitive radioimmunoassay (cRIA) and for neutralization by use of the athymic mouse xenograft system. Analysis of serum from 104 subjects showed a dose response in HPV-11 cRIA titers and neutralization. Overall, 68 (82.9%) of 82 postimmunization serum specimens from VLP recipients were 100% neutralizing when used in the assay at a 1:50 dilution. Of 69 serum specimens, 63 (91.3%) with cRIA titers >200 milliMerck units per milliliter were neutralizing. Immunization with HPV VLPs elicits a vigorous serum immune response in a high percentage of women. The HPV-11 cRIA titer appears to be a surrogate marker for neutralization.Entities:
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Year: 2001 PMID： 11598842 DOI： 10.1086/323645
Source DB: PubMed Journal: J Infect Dis ISSN： 0022-1899 Impact factor: 5.226