OBJECTIVE: The purpose of the present investigation was to examine the microbiota of acute periradicular abscesses of endodontic origin by using a molecular genetic method. STUDY DESIGN: Pus was collected by aspiration from 27 cases diagnosed as acute abscesses of endodontic origin, and DNA was extracted to evaluate the occurrence of 49 bacterial species by using whole genomic DNA probes and checkerboard DNA-DNA hybridization. The presence of bacterial DNA in clinical samples was confirmed by polymerase chain reaction with ubiquitous bacterial 16S rRNA gene primers. RESULTS: The results of the checkerboard DNA-DNA hybridization analysis revealed that 37 of the 49 DNA probes tested were reactive with one or more samples. The number of bacterial species in the pus samples ranged from 1 to 33 (mean, 5.9). Eighteen of the 27 pus samples were positive for at least one DNA probe. The most prevalent species found were: Bacteroides forsythus (29.6% of the cases); Porphyromonas gingivalis (29.6%); Streptococcus constellatus (25.9%), Prevotella intermedia (22.2%), Prevotella nigrescens (22.2%), Fusobacterium periodonticum (18.5%), Fusobacterium nucleatum subspecies nucleatum (18.5%), and Eikenella corrodens (18.5%). CONCLUSIONS: The microbiologic data of the present investigation indicated that molecular genetic methods could provide additional knowledge regarding the microbiota of acute periradicular abscesses by detecting bacterial species that are difficult--or even impossible--to grow.
OBJECTIVE: The purpose of the present investigation was to examine the microbiota of acute periradicular abscesses of endodontic origin by using a molecular genetic method. STUDY DESIGN: Pus was collected by aspiration from 27 cases diagnosed as acute abscesses of endodontic origin, and DNA was extracted to evaluate the occurrence of 49 bacterial species by using whole genomic DNA probes and checkerboard DNA-DNA hybridization. The presence of bacterial DNA in clinical samples was confirmed by polymerase chain reaction with ubiquitous bacterial 16S rRNA gene primers. RESULTS: The results of the checkerboard DNA-DNA hybridization analysis revealed that 37 of the 49 DNA probes tested were reactive with one or more samples. The number of bacterial species in the pus samples ranged from 1 to 33 (mean, 5.9). Eighteen of the 27 pus samples were positive for at least one DNA probe. The most prevalent species found were: Bacteroides forsythus (29.6% of the cases); Porphyromonas gingivalis (29.6%); Streptococcus constellatus (25.9%), Prevotella intermedia (22.2%), Prevotella nigrescens (22.2%), Fusobacterium periodonticum (18.5%), Fusobacterium nucleatum subspecies nucleatum (18.5%), and Eikenella corrodens (18.5%). CONCLUSIONS: The microbiologic data of the present investigation indicated that molecular genetic methods could provide additional knowledge regarding the microbiota of acute periradicular abscesses by detecting bacterial species that are difficult--or even impossible--to grow.
Authors: E L R Sousa; B P F A Gomes; R C Jacinto; A A Zaia; C C R Ferraz Journal: Eur J Clin Microbiol Infect Dis Date: 2012-12-06 Impact factor: 3.267
Authors: L C N Brito; F R Teles; R P Teles; E C França; A P Ribeiro-Sobrinho; A D Haffajee; S S Socransky Journal: J Clin Microbiol Date: 2007-07-18 Impact factor: 5.948
Authors: Julio C Machado de Oliveira; Tulio G V Gama; José F Siqueira; Isabela N Rôças; Raquel S Peixoto; Alexandre S Rosado Journal: Clin Oral Investig Date: 2006-11-08 Impact factor: 3.606
Authors: Sebastian Böttger; Silke Zechel-Gran; Daniel Schmermund; Philipp Streckbein; Jan-Falco Wilbrand; Michael Knitschke; Jörn Pons-Kühnemann; Torsten Hain; Markus Weigel; Hans-Peter Howaldt; Eugen Domann; Sameh Attia Journal: Microorganisms Date: 2021-06-16