OBJECTIVE: To prepare, sequence and analyse adult human cartilage cDNA libraries to study the gene expression pattern between normal and osteoarthritic cartilage. METHODS: Poly A(+)RNA from adult human normal and osteoarthritic articular cartilage was isolated and used to prepare cDNA libraries. Approximately 5000 ESTs from each library were sequenced and analysed using bioinformatic tools. The expression of select genes was confirmed by Northern blot and in situ hybridization analysis. RESULTS: Multiple gene families including several classical cartilage matrix protein encoding genes were identified. Approximately 28-40% of the genes sequenced from these libraries were novel, while half of the genes encoded known proteins and 4-6% of the genes encoded novel homologs of known proteins. Several known genes, whose expression has not been reported previously in cartilage, were also identified. We have confirmed the cartilage expression of three known (CTGF, CTGF-L and clusterin) and two novel homologs of known genes (PCPE-2 and Gal-Nac transferase) by Northern blot and in situ hybridization analysis. CONCLUSION: This is the first report of the preparation and sequencing of cDNA libraries from adult human normal and osteoarthritic articular cartilage. Further analysis of genes identified from these libraries may provide molecular targets for diagnosis and/or treatment of osteoarthritis (OA). Copyright 2001 OsteoArthritis Research Society International.
OBJECTIVE: To prepare, sequence and analyse adult humancartilage cDNA libraries to study the gene expression pattern between normal and osteoarthritic cartilage. METHODS: Poly A(+)RNA from adult human normal and osteoarthritic articular cartilage was isolated and used to prepare cDNA libraries. Approximately 5000 ESTs from each library were sequenced and analysed using bioinformatic tools. The expression of select genes was confirmed by Northern blot and in situ hybridization analysis. RESULTS: Multiple gene families including several classical cartilage matrix protein encoding genes were identified. Approximately 28-40% of the genes sequenced from these libraries were novel, while half of the genes encoded known proteins and 4-6% of the genes encoded novel homologs of known proteins. Several known genes, whose expression has not been reported previously in cartilage, were also identified. We have confirmed the cartilage expression of three known (CTGF, CTGF-L and clusterin) and two novel homologs of known genes (PCPE-2 and Gal-Nac transferase) by Northern blot and in situ hybridization analysis. CONCLUSION: This is the first report of the preparation and sequencing of cDNA libraries from adult human normal and osteoarthritic articular cartilage. Further analysis of genes identified from these libraries may provide molecular targets for diagnosis and/or treatment of osteoarthritis (OA). Copyright 2001 OsteoArthritis Research Society International.
Authors: Melissa S Ashwell; Michael G Gonda; Kent Gray; Christian Maltecca; Audrey T O'Nan; Joseph P Cassady; Peter L Mente Journal: J Orthop Res Date: 2012-10-01 Impact factor: 3.494
Authors: Maria D Mayan; Paula Carpintero-Fernandez; Raquel Gago-Fuentes; Oskar Martinez-de-Ilarduya; Hong-Zhang Wang; Virginijus Valiunas; Peter Brink; Francisco J Blanco Journal: Am J Pathol Date: 2013-02-13 Impact factor: 4.307
Authors: Jose A Morcuende; Xiao Dong Huang; Jeff Stevens; Tammy A Kucaba; Bart Brown; Hakeem Abdulkawy; Todd E Scheetz; Sergey Malchenko; Fatima Bonaldo; Thomas L Casavant; Bento Soares Journal: Iowa Orthop J Date: 2002
Authors: Jose A Morcuende; Jeff W Stevens; Todd E Scheetz; Maria de Fatima Bonaldoc; Thomas L Casavant; Jesse E Otero; Marcelo B Soares Journal: Iowa Orthop J Date: 2012
Authors: Gillian Murphy; Vera Knäuper; Susan Atkinson; George Butler; William English; Mike Hutton; Jan Stracke; Ian Clark Journal: Arthritis Res Date: 2002-05-09