AIMS/HYPOTHESIS: We describe a new Type I (insulin-dependent) diabetes mellitus rat model (LEW.1AR1/Ztm-iddm) which arose through a spontaneous mutation in a congenic Lewis rat strain with a defined MHC haplotype (RT1.Aa B/Du Cu). METHODS: The development of diabetes was characterised using biochemical, immunological and morphological methods. RESULTS: Diabetes appeared in the rats with an incidence of 20 % without major sex preference at 58+/-2 days. The disease was characterised by hyperglycaemia, glycosuria, ketonuria and polyuria. In peripheral blood, the proportion of T lymphocytes was in the normal range expressing the RT6.1 differentiation antigen. Islets were heavily infiltrated with B and T lymphocytes, macrophages and NK cells with beta cells rapidly destroyed through apoptosis in areas of insulitis. CONCLUSION/ INTERPRETATION: This Type I diabetic rat develops a spontaneous insulin-dependent autoimmune diabetes through beta cell apoptosis. It could prove to be a valuable new animal model for clarifying the mechanisms involved in the development of autoimmune diabetes.
AIMS/HYPOTHESIS: We describe a new Type I (insulin-dependent) diabetes mellitusrat model (LEW.1AR1/Ztm-iddm) which arose through a spontaneous mutation in a congenic Lewis rat strain with a defined MHC haplotype (RT1.Aa B/Du Cu). METHODS: The development of diabetes was characterised using biochemical, immunological and morphological methods. RESULTS:Diabetes appeared in the rats with an incidence of 20 % without major sex preference at 58+/-2 days. The disease was characterised by hyperglycaemia, glycosuria, ketonuria and polyuria. In peripheral blood, the proportion of T lymphocytes was in the normal range expressing the RT6.1 differentiation antigen. Islets were heavily infiltrated with B and T lymphocytes, macrophages and NK cells with beta cells rapidly destroyed through apoptosis in areas of insulitis. CONCLUSION/ INTERPRETATION: This Type I diabeticrat develops a spontaneous insulin-dependent autoimmune diabetes through beta cell apoptosis. It could prove to be a valuable new animal model for clarifying the mechanisms involved in the development of autoimmune diabetes.
Authors: Elizabeth P Rakoczy; Ireni S Ali Rahman; Nicolette Binz; Cai-Rui Li; Nermina N Vagaja; Marisa de Pinho; Chooi-May Lai Journal: Am J Pathol Date: 2010-09-09 Impact factor: 4.307
Authors: J M Fuller; M Bogdani; T D Tupling; R A Jensen; R Pefley; S Manavi; L Cort; E P Blankenhorn; J P Mordes; A Lernmark; A E Kwitek Journal: Physiol Genomics Date: 2009-04-07 Impact factor: 3.107
Authors: Rebecca S Tirabassi; Dennis L Guberski; Elizabeth P Blankenhorn; Jean H Leif; Bruce A Woda; Zhijun Liu; Donald Winans; Dale L Greiner; John P Mordes Journal: Diabetes Date: 2009-09-30 Impact factor: 9.461