PURPOSE: To identify localization and kinetics of infiltrated cells and cytokines in murine herpetic keratitis. METHODS: HSV-1 was inoculated onto the scarified BALB/c corneas. At given times post infection (PI), eyes were removed and studied immunohistochemically using monoclonal antibodies against several infiltrated cells and cytokines. RESULTS: Neutrophils and NK cells infiltrated as early as 1 day PI reaching a maximum number at 2 day PI in initial stage. gamma delta TCR positive cells were observed in the corneal stroma from 1 day PI to 8 day PI. IL-2 and IFN-gamma were positive in the cell-infiltrated areas of the epithelial and stromal lesions, whereas IL-4 was negative throughout the experiment. CONCLUSION: Our results indicated that cytokine profile upon herpes infection on the cornea is Th1 dominant. Together with neutrophils in the early phase of infection, gamma delta positive T cells may play an additional role in protecting the cornea against incoming pathogens.
PURPOSE: To identify localization and kinetics of infiltrated cells and cytokines in murineherpetic keratitis. METHODS:HSV-1 was inoculated onto the scarified BALB/c corneas. At given times post infection (PI), eyes were removed and studied immunohistochemically using monoclonal antibodies against several infiltrated cells and cytokines. RESULTS: Neutrophils and NK cells infiltrated as early as 1 day PI reaching a maximum number at 2 day PI in initial stage. gamma delta TCR positive cells were observed in the corneal stroma from 1 day PI to 8 day PI. IL-2 and IFN-gamma were positive in the cell-infiltrated areas of the epithelial and stromal lesions, whereas IL-4 was negative throughout the experiment. CONCLUSION: Our results indicated that cytokine profile upon herpes infection on the cornea is Th1 dominant. Together with neutrophils in the early phase of infection, gamma delta positive T cells may play an additional role in protecting the cornea against incoming pathogens.
Authors: Heather M Cathcart; Mei Zheng; Jason J Covar; Yi Liu; Robert Podolsky; Sally S Atherton Journal: Invest Ophthalmol Vis Sci Date: 2011-06-07 Impact factor: 4.799
Authors: Sharon Altmann; Megan Toomey; Brittany Nesbit; Kim McIntyre; Jill Covert; Richard Redd Dubielzig; Gary Leatherberry; Elizabeth Adkins; Christopher J Murphy; Curtis R Brandt Journal: Invest Ophthalmol Vis Sci Date: 2010-04-07 Impact factor: 4.799
Authors: Sohyun Jeon; Alexander M Rowe; Kate L Carroll; Stephen A K Harvey; Robert L Hendricks Journal: J Immunol Date: 2018-04-18 Impact factor: 5.422