X You1, W Teng, Z Shan. 1. Department of Internal Medicine, Peking Union Medical College Hospital, Beijing 100730, China.
Abstract
OBJECTIVE: To detect expression of intercellular adhesion molecule-1 (ICAM-1), B7.1 and thyroid peroxidase (TPO) on thyrocyte and study the possible mechanism of interferon-alpha (IFN-alpha) in the pathogenesis of autoimmune thyroid disease (AITD). METHODS: Thyrocytes were cultured from 6 normal persons. Antigen expression on thyrocytes induced by cytokines was examined using immunofluorescence staining with flow cytometer. RESULTS: IFN-alpha significantly stimulated the expression of ICAM-1, B7.1 and TPO, as compared with those of control group. IFN-gamma markedly enhanced the expression of HLA-DR and ICAM-1, but not B7.1. Prolactin (PRL) resulted in increased expression of ICAM-1, B7.1, as well as overexpression of TPO, which is more significant than that stimulated by IFN-alpha. CONCLUSIONS: Thyroid autoimmunity induced by IFN-alpha is associated with the expression of ICAM-1, B7.1 and TPO. IFN-gamma could not induce the expression of B7.1, therefore it is not an initiator in AITD. In addition, we should pay more attention to PRL which possibly plays an important role in the initiation and perpetuation of postpartum thyroiditis.
OBJECTIVE: To detect expression of intercellular adhesion molecule-1 (ICAM-1), B7.1 and thyroid peroxidase (TPO) on thyrocyte and study the possible mechanism of interferon-alpha (IFN-alpha) in the pathogenesis of autoimmune thyroid disease (AITD). METHODS: Thyrocytes were cultured from 6 normal persons. Antigen expression on thyrocytes induced by cytokines was examined using immunofluorescence staining with flow cytometer. RESULTS:IFN-alpha significantly stimulated the expression of ICAM-1, B7.1 and TPO, as compared with those of control group. IFN-gamma markedly enhanced the expression of HLA-DR and ICAM-1, but not B7.1. Prolactin (PRL) resulted in increased expression of ICAM-1, B7.1, as well as overexpression of TPO, which is more significant than that stimulated by IFN-alpha. CONCLUSIONS: Thyroid autoimmunity induced by IFN-alpha is associated with the expression of ICAM-1, B7.1 and TPO. IFN-gamma could not induce the expression of B7.1, therefore it is not an initiator in AITD. In addition, we should pay more attention to PRL which possibly plays an important role in the initiation and perpetuation of postpartum thyroiditis.
Authors: Trevor E Angell; Melissa G Lechner; Julie K Jang; Jonathan S LoPresti; Alan L Epstein Journal: Clin Cancer Res Date: 2014-10-07 Impact factor: 12.531