Literature DB >> 11592841

A single-step affinity column for purification of serotype-5 based adeno-associated viral vectors.

A Auricchio1, E O'Connor, M Hildinger, J M Wilson.   

Abstract

Here we describe a single-step affinity column for purification of vectors based on adeno-associated virus type 5 (AAV5). A sialic-acid-rich protein called mucin was covalently attached to Sepharose and was found to bind AAV5 vectors. Elution with high salt efficiently recovered highly active vectors of greater purity than what is achieved with CsCl(2) sedimentation.

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Year:  2001        PMID： 11592841     DOI： 10.1006/mthe.2001.0462

Source DB:  PubMed          Journal:  Mol Ther        ISSN： 1525-0016            Impact factor:   11.454


  17 in total

1.  Cross-dressing the virion: the transcapsidation of adeno-associated virus serotypes functionally defines subgroups.

Authors:  Joseph E Rabinowitz; Dawn E Bowles; Susan M Faust; Julie G Ledford; Scott E Cunningham; R Jude Samulski
Journal:  J Virol       Date:  2004-05       Impact factor: 5.103

2.  Novel AAV serotypes for improved ocular gene transfer.

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Journal:  J Gene Med       Date:  2008-04       Impact factor: 4.565

3.  Overcoming the cystic fibrosis sputum barrier to leading adeno-associated virus gene therapy vectors.

Authors:  Benjamin S Schuster; Anthony J Kim; Joshua C Kays; Mia M Kanzawa; William B Guggino; Michael P Boyle; Steven M Rowe; Nicholas Muzyczka; Jung Soo Suk; Justin Hanes
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Review 4.  AAV hybrid serotypes: improved vectors for gene delivery.

Authors:  Vivian W Choi; Douglas M McCarty; R Jude Samulski
Journal:  Curr Gene Ther       Date:  2005-06       Impact factor: 4.391

5.  Efficient AAV1-AAV2 hybrid vector for gene therapy of hemophilia.

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6.  Impact of preexisting vector immunity on the efficacy of adeno-associated virus-based HIV-1 Gag vaccines.

Authors:  Jianping Lin; Roberto Calcedo; Luk H Vandenberghe; Joanita M Figueredo; James M Wilson
Journal:  Hum Gene Ther       Date:  2008-07       Impact factor: 5.695

7.  Local and systemic responses following intravitreous injection of AAV2-encoded modified Volvox channelrhodopsin-1 in a genetically blind rat model.

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8.  Non-erythropoietic erythropoietin derivatives protect from light-induced and genetic photoreceptor degeneration.

Authors:  Pasqualina Colella; Carolina Iodice; Umberto Di Vicino; Ida Annunziata; Enrico M Surace; Alberto Auricchio
Journal:  Hum Mol Genet       Date:  2011-03-19       Impact factor: 6.150

9.  Adeno-Associated Virus-Mediated Gene Transfer to Renal Tubule Cells via a Retrograde Ureteral Approach.

Authors:  Daniel C Chung; Ben Fogelgren; Kwon Moo Park; Jessica Heidenberg; Xiaofeng Zuo; Liwei Huang; Jean Bennett; Joshua H Lipschutz
Journal:  Nephron Extra       Date:  2011-11-23

10.  Adeno-associated virus-mediated gene delivery into the scala media of the normal and deafened adult mouse ear.

Authors:  L A Kilpatrick; Q Li; J Yang; J C Goddard; D M Fekete; H Lang
Journal:  Gene Ther       Date:  2011-01-06       Impact factor: 5.250
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