G I Gorodeski1. 1. Department of Obstetrics and Gynecology, University MacDonald Women's Hospital, Cleveland, Ohio 44106, USA. gig@po.cwru.edu
Abstract
OBJECTIVE: To determine the effects of menopause (aging and E) on vaginal-cervical epithelial paracellular permeability. DESIGN: Experimental, basic clinical research. SETTING: Academic research environment. PATIENT(S): Premenopausal, perimenopausal, and postmenopausal women, aged 35-65 years. INTERVENTION(S): Primary to tertiary cultures of normal human ectocervical epithelial cells on filters. Cells were outgrown from surgically discarded ectocervical minces. MAIN OUTCOME MEASURE(S): Changes in paracellular permeability were determined as changes in transepithelial electrical conductance and pyranine permeability. RESULT(S): [1] Levels of transepithelial electrical conductance and pyranine permeability decreased as women's age advanced. [2] Removal of E from the culture medium decreased paracellular permeability. Treatment of cells in vitro with 10 nmol/L 17beta-E2 increased transepithelial electrical conductance and pyranine permeability, but the effects were additive to the age-related decrease in permeability. [3] Coadministration of 100 nmol/L tamoxifen blocked the E increase in paracellular permeability in cells of both premenopausal and postmenopausal women. CONCLUSION(S): [1] Aging and E deficiency decrease independently vaginal-cervical epithelial paracellular permeability. [2] The E increase in vaginal-cervical epithelial paracellular permeability in cells of postmenopausal women is mediated by the E receptor. [3] The E increase in vaginal-cervical epithelial paracellular permeability in cells of postmenopausal women is masked by age-related increase in the tight junctional resistance, leading to overall decrease in paracellular permeability.
OBJECTIVE: To determine the effects of menopause (aging and E) on vaginal-cervical epithelial paracellular permeability. DESIGN: Experimental, basic clinical research. SETTING: Academic research environment. PATIENT(S): Premenopausal, perimenopausal, and postmenopausal women, aged 35-65 years. INTERVENTION(S): Primary to tertiary cultures of normal human ectocervical epithelial cells on filters. Cells were outgrown from surgically discarded ectocervical minces. MAIN OUTCOME MEASURE(S): Changes in paracellular permeability were determined as changes in transepithelial electrical conductance and pyranine permeability. RESULT(S): [1] Levels of transepithelial electrical conductance and pyranine permeability decreased as women's age advanced. [2] Removal of E from the culture medium decreased paracellular permeability. Treatment of cells in vitro with 10 nmol/L 17beta-E2 increased transepithelial electrical conductance and pyranine permeability, but the effects were additive to the age-related decrease in permeability. [3] Coadministration of 100 nmol/L tamoxifen blocked the E increase in paracellular permeability in cells of both premenopausal and postmenopausal women. CONCLUSION(S): [1] Aging and E deficiency decrease independently vaginal-cervical epithelial paracellular permeability. [2] The E increase in vaginal-cervical epithelial paracellular permeability in cells of postmenopausal women is mediated by the E receptor. [3] The E increase in vaginal-cervical epithelial paracellular permeability in cells of postmenopausal women is masked by age-related increase in the tight junctional resistance, leading to overall decrease in paracellular permeability.
Authors: Silke Baldewijns; Mart Sillen; Ilse Palmans; Paul Vandecruys; Patrick Van Dijck; Liesbeth Demuyser Journal: Front Microbiol Date: 2021-07-02 Impact factor: 5.640
Authors: Lyndsey R Buckner; Angela M Amedee; Hannah L Albritton; Pamela A Kozlowski; Nedra Lacour; Chris L McGowin; Danny J Schust; Alison J Quayle Journal: PLoS One Date: 2016-01-05 Impact factor: 3.240