Literature DB >> 11590598

Expression of recombinant human acetylcholinesterase in transgenic tomato plants.

T S Mor1, M Sternfeld, H Soreq, C J Arntzen, H S Mason.   

Abstract

Enzyme therapy for the prevention and treatment of organophosphate poisoning depends on the availability of large amounts of cholinesterases. Transgenic plants are being evaluated for their efficiency and cost-effectiveness as a system for the bioproduction of therapeutically valuable proteins. Here we report production of a recombinant isoform of human acetylcholinesterase in transgenic tomato plants. Active and stable acetylcholinesterase, which retains the kinetic characteristics of the human enzyme, accumulated in tomato plants. High levels of specific activity were registered in leaves (up to 25 nmol min(-1) mg protein(-1)) and fruits (up to 250 nmol min(-1) mg protein(-1)). Copyright 2001 John Wiley & Sons, Inc.

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Year:  2001        PMID： 11590598     DOI： 10.1002/bit.10012

Source DB:  PubMed          Journal:  Biotechnol Bioeng        ISSN： 0006-3592            Impact factor:   4.530


  16 in total

1.  Tissue distribution of cholinesterases and anticholinesterases in native and transgenic tomato plants.

Authors:  Samuel P Fletcher; Brian C Geyer; Amy Smith; Tama Evron; Lokesh Joshi; Hermona Soreq; Tsafrir S Mor
Journal:  Plant Mol Biol       Date:  2004-05       Impact factor: 4.076

2.  The Arabidopsis thaliana ortholog of a purported maize cholinesterase gene encodes a GDSL-lipase.

Authors:  Mrinalini Muralidharan; Kristina Buss; Katherine E Larrimore; Nicholas A Segerson; Latha Kannan; Tsafrir S Mor
Journal:  Plant Mol Biol       Date:  2013-02-22       Impact factor: 4.076

Review 3.  Microalgae as bioreactors.

Authors:  Tara L Walker; Saul Purton; Douglas K Becker; Chris Collet
Journal:  Plant Cell Rep       Date:  2005-08-31       Impact factor: 4.570

4.  Plant-derived human acetylcholinesterase-R provides protection from lethal organophosphate poisoning and its chronic aftermath.

Authors:  Tama Evron; Brian C Geyer; Irene Cherni; Mrinalini Muralidharan; Jacquelyn Kilbourne; Samuel P Fletcher; Hermona Soreq; Tsafrir S Mor
Journal:  FASEB J       Date:  2007-05-02       Impact factor: 5.191

5.  Transgenic tomato expressing interleukin-12 has a therapeutic effect in a murine model of progressive pulmonary tuberculosis.

Authors:  A L Elías-López; B Marquina; A Gutiérrez-Ortega; D Aguilar; M Gomez-Lim; R Hernández-Pando
Journal:  Clin Exp Immunol       Date:  2008-08-22       Impact factor: 4.330

6.  Expression of functional interleukin-12 from mouse in transgenic tomato plants.

Authors:  Abel Gutiérrez-Ortega; Claudia Sandoval-Montes; Teresa Jesús de Olivera-Flores; Leopoldo Santos-Argumedo; Miguel Angel Gómez-Lim
Journal:  Transgenic Res       Date:  2005-12       Impact factor: 3.145

Review 7.  The Potential for Microalgae as Bioreactors to Produce Pharmaceuticals.

Authors:  Na Yan; Chengming Fan; Yuhong Chen; Zanmin Hu
Journal:  Int J Mol Sci       Date:  2016-06-17       Impact factor: 5.923

8.  Hairy-root organ cultures for the production of human acetylcholinesterase.

Authors:  Ryan R Woods; Brian C Geyer; Tsafrir S Mor
Journal:  BMC Biotechnol       Date:  2008-12-23       Impact factor: 2.563

9.  Biological and biochemical characterization of HIV-1 Gag/dgp41 virus-like particles expressed in Nicotiana benthamiana.

Authors:  Sarah A Kessans; Mark D Linhart; Nobuyuki Matoba; Tsafrir Mor
Journal:  Plant Biotechnol J       Date:  2013-03-19       Impact factor: 9.803

10.  Translational control of recombinant human acetylcholinesterase accumulation in plants.

Authors:  Brian C Geyer; Samuel P Fletcher; Tagan A Griffin; Michael J Lopker; Hermona Soreq; Tsafrir S Mor
Journal:  BMC Biotechnol       Date:  2007-05-30       Impact factor: 2.563
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