| Literature DB >> 11587135 |
R W Wulkan1, R Verwers, M Neele, M J Mantel.
Abstract
Accurate measurement of pyruvate is particularly important in the detection of mitochondrial enzyme disorders. describe a simple high-performance liquid chromatography assay for pyruvate with derivatization and fluorescence detection. Between-run variation is below 4.5% and the method is linear between I and 1,200 micromol/L. The mean recovery is 101%. After sample pretreatment, the calculated pyruvate concentration proved to be stable for 15 days when samples were kept at -20 degrees C.Entities:
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Year: 2001 PMID： 11587135 DOI： 10.1177/000456320103800514
Source DB: PubMed Journal: Ann Clin Biochem ISSN： 0004-5632 Impact factor: 2.057