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Literature DB >> 11578933

Detection of protein-protein interactions in vivo based on protein splicing.

Abstract

In mammalian cells, protein-protein interactions constitute essential regulatory steps that modulate the activity of signaling pathways. In recent years, several approaches towards understanding the interactions have been developed. We describe herein a new method for detecting protein-protein interactions in vivo based on protein splicing and highlight some potential applications of this technique.

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Year: 2001 PMID： 11578933 DOI： 10.1016/s1367-5931(00)00244-1

Source DB: PubMed Journal: Curr Opin Chem Biol ISSN： 1367-5931 Impact factor: 8.822

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Cited

10 in total

1. Assembling a Correctly Folded and Functional Heptahelical Membrane Protein by Protein Trans-splicing.

Authors: Michaela Mehler; Carl Elias Eckert; Alena Busche; Jennifer Kulhei; Jonas Michaelis; Johanna Becker-Baldus; Josef Wachtveitl; Volker Dötsch; Clemens Glaubitz
Journal: J Biol Chem Date: 2015-09-24 Impact factor: 5.157

2. Free energies of protein-protein association determined by electrospray ionization mass spectrometry correlate accurately with values obtained by solution methods.

Authors: Sanjay R Krishnaswamy; Evan R Williams; Jack F Kirsch
Journal: Protein Sci Date: 2006-06 Impact factor: 6.725

3. Utp8p is a nucleolar tRNA-binding protein that forms a complex with components of the nuclear tRNA export machinery in Saccharomyces cerevisiae.

Authors: Benjamin R Strub; Manoja B K Eswara; Jacqueline B Pierce; Dev Mangroo
Journal: Mol Biol Cell Date: 2007-07-18 Impact factor: 4.138

4. A versatile amino acid analogue of the solvatochromic fluorophore 4-N,N-dimethylamino-1,8-naphthalimide: a powerful tool for the study of dynamic protein interactions.

Authors: Galen Loving; Barbara Imperiali
Journal: J Am Chem Soc Date: 2008-09-23 Impact factor: 15.419

Review 10. NBD-based synthetic probes for sensing small molecules and proteins: design, sensing mechanisms and biological applications.

Authors: Chenyang Jiang; Haojie Huang; Xueying Kang; Liu Yang; Zhen Xi; Hongyan Sun; Michael D Pluth; Long Yi
Journal: Chem Soc Rev Date: 2021-07-05 Impact factor: 60.615

10 in total

Detection of protein-protein interactions in vivo based on protein splicing.

1. Assembling a Correctly Folded and Functional Heptahelical Membrane Protein by Protein Trans-splicing.

2. Free energies of protein-protein association determined by electrospray ionization mass spectrometry correlate accurately with values obtained by solution methods.

3. Utp8p is a nucleolar tRNA-binding protein that forms a complex with components of the nuclear tRNA export machinery in Saccharomyces cerevisiae.

4. A versatile amino acid analogue of the solvatochromic fluorophore 4-N,N-dimethylamino-1,8-naphthalimide: a powerful tool for the study of dynamic protein interactions.

5. Time-lapse imaging of a dynamic phosphorylation-dependent protein-protein interaction in mammalian cells.

6. Balboa binds to pickpocket in vivo and is required for mechanical nociception in Drosophila larvae.

7. Dual-color click beetle luciferase heteroprotein fragment complementation assays.

Review 8. Bioluminescence imaging: progress and applications.

9. Complementation and reconstitution of fluorescence from circularly permuted and truncated green fluorescent protein.

Review 10. NBD-based synthetic probes for sensing small molecules and proteins: design, sensing mechanisms and biological applications.