Literature DB >> 11570843

High-level expression and purification of immunogenic recombinant SAG1 (P30) of Toxoplasma gondii in Escherichia coli.

X G Chen1, Y Gong, Z R Lun, M C Fung.   

Abstract

Surface antigen 1 (SAG1) of Toxoplasma gondii is a good candidate for diagnosis and vaccine development, but recombinant SAG1 produced in Escheichia coli often loses its specific immunogenicity due to the incorrect folding. In the present study, a truncated SAG1 was highly expressed in E. coli as a fusion protein, about 30% of the total protein of the cell lysate. After a simple purification and refolding procedure, purified rSAG1 can be recognized by human Toxoplasma-infective serum, and ELISA kits constructed by rSAG1 can sensitively and specifically detect toxoplasma infection. Copyright 2001 Academic Press.

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Year:  2001        PMID： 11570843     DOI： 10.1006/prep.2001.1483

Source DB:  PubMed          Journal:  Protein Expr Purif        ISSN： 1046-5928            Impact factor:   1.650


  12 in total

1.  High-level expression and purification of a truncated merozoite antigen-2 of Babesia equi in Escherichia coli and its potential for immunodiagnosis.

Authors:  Xiaohong Huang; Xuenan Xuan; Naoaki Yokoyama; Longshan Xu; Hiroshi Suzuki; Chihiro Sugimoto; Hideyuki Nagasawa; Kozo Fujisaki; Ikuo Igarashi
Journal:  J Clin Microbiol       Date:  2003-03       Impact factor: 5.948

2.  Production of the main surface antigen of Toxoplasma gondii in tobacco leaves and analysis of its antigenicity and immunogenicity.

Authors:  Marina Clemente; Roberto Curilovic; Alina Sassone; Alicia Zelada; Sergio O Angel; Alejandro N Mentaberry
Journal:  Mol Biotechnol       Date:  2005-05       Impact factor: 2.695

3.  Identification of a sporozoite-specific antigen from Toxoplasma gondii.

Authors:  Dolores Hill; Cathleen Coss; J P Dubey; Kristen Wroblewski; Mari Sautter; Tiffany Hosten; Claudia Muñoz-Zanzi; Ernest Mui; Shawn Withers; Kenneth Boyer; Gretchen Hermes; Jessica Coyne; Frank Jagdis; Andrew Burnett; Patrick McLeod; Holmes Morton; Donna Robinson; Rima McLeod
Journal:  J Parasitol       Date:  2011-03-03       Impact factor: 1.276

4.  Enzyme-linked immunosorbent assay using recombinant SAG1 antigen to detect Toxoplasma gondii-specific immunoglobulin G antibodies in human sera and saliva.

Authors:  Nouha Chahed Bel-Ochi; Aïda Bouratbine; Mohamed Mousli
Journal:  Clin Vaccine Immunol       Date:  2013-01-23

Review 5.  Advances in serological, imaging techniques and molecular diagnosis of Toxoplasma gondii infection.

Authors:  Ali Rostami; Panagiotis Karanis; Shirzad Fallahi
Journal:  Infection       Date:  2018-01-12       Impact factor: 3.553

Review 6.  Toxoplasma gondii recombinant antigens as tools for serodiagnosis of human toxoplasmosis: current status of studies.

Authors:  Lucyna Holec-Gasior
Journal:  Clin Vaccine Immunol       Date:  2013-06-19

7.  Recombinant SAG1 Antigen to Detect Toxoplasma gondii Specific Immunoglobulin G in Human Sera by ELISA Test.

Authors:  N Jalallou; M Bandepour; H Khazan; A Haghighi; Sh Abdollahi; B Kazemi
Journal:  Iran J Parasitol       Date:  2010-06       Impact factor: 1.012

8.  Cloning and Expression of Major Surface Antigen 1 Gene of Toxoplasma gondii RH Strain Using the Expression Vector pVAX1 in Chinese Hamster Ovary Cells.

Authors:  Rahman Abdizadeh; Sharif Maraghi; Ata A Ghadiri; Mehdi Tavalla; Saeedeh Shojaee
Journal:  Jundishapur J Microbiol       Date:  2015-03-21       Impact factor: 0.747

9.  Production and Evaluation of Toxoplasma gondii Recombinant Surface Antigen 1 (SAG1) for Serodiagnosis of Acute and Chronic Toxoplasma Infection in Human Sera.

Authors:  M Mina Selseleh; H Keshavarz; M Mohebali; S Shojaee; Mh Modarressi; Mr Eshragian; M Monavar Selseleh
Journal:  Iran J Parasitol       Date:  2012       Impact factor: 1.012

Review 10.  Research progress on surface antigen 1 (SAG1) of Toxoplasma gondii.

Authors:  Yanhua Wang; Hong Yin
Journal:  Parasit Vectors       Date:  2014-04-13       Impact factor: 3.876
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