Role of chicken serum in inhibiting Leucocytozoon caulleryi development in Culicoides arakawae infected by membrane-feeding of infective blood meals.

Leucocytozoon caulleryi, the most important pathogen of leucocytozoonsis in several Asian countries, is transmitted by Culicoides arakawae. Although the vector blood meal factors that influence the infectivity of L. caulleryi remain poorly understood, the factors in infected chicken serum remain vital. C. arakawae blood-fed through a membrane were used in this experiment to examine the influence of serum factors on L. caulleryi development. Experimental results indicate that C. arakawae were successfully blood-fed through a chicken-egg-shell membrane and the serum factors in infected chicks significantly affected L. caulleryi sporogony. The inhibition effect of serum factors calculated from sporozoite averages was 53.6% +/- 2.2%. The serum factors, including transmission-blocking antibody, require further detailed study.