Literature DB >> 11569803

A bacterial method for the nitrogen isotopic analysis of nitrate in seawater and freshwater.

D M Sigman1, K L Casciotti, M Andreani, C Barford, M Galanter, J K Böhlke.   

Abstract

We report a new method for measurement of the isotopic composition of nitrate (NO3-) at the natural-abundance level in both seawater and freshwater. The method is based on the isotopic analysis of nitrous oxide (N20) generated from nitrate by denitrifying bacteria that lack N2O-reductase activity. The isotopic composition of both nitrogen and oxygen from nitrate are accessible in this way. In this first of two companion manuscripts, we describe the basic protocol and results for the nitrogen isotopes. The precision of the method is better than 0.2/1000 (1 SD) at concentrations of nitrate down to 1 microM, and the nitrogen isotopic differences among various standards and samples are accurately reproduced. For samples with 1 microM nitrate or more, the blank of the method is less than 10% of the signal size, and various approaches may reduce it further.

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Year:  2001        PMID： 11569803     DOI： 10.1021/ac010088e

Source DB:  PubMed          Journal:  Anal Chem        ISSN： 0003-2700            Impact factor:   6.986


  105 in total

1.  Isotopic signals of summer denitrification in a northern hardwood forested catchment.

Authors:  Sarah K Wexler; Christine L Goodale; Kevin J McGuire; Scott W Bailey; Peter M Groffman
Journal:  Proc Natl Acad Sci U S A       Date:  2014-11-03       Impact factor: 11.205

2.  Nutrient regime shift in the western North Atlantic indicated by compound-specific δ15N of deep-sea gorgonian corals.

Authors:  Owen A Sherwood; Moritz F Lehmann; Carsten J Schubert; David B Scott; Matthew D McCarthy
Journal:  Proc Natl Acad Sci U S A       Date:  2011-01-03       Impact factor: 11.205

3.  Reservoir sediments: a sink or source of chemicals at the surface water-groundwater interface.

Authors:  Rawaa Ammar; Véronique Kazpard; Mahmoud Wazne; Antoine G El Samrani; Nabil Amacha; Zeinab Saad; Lei Chou
Journal:  Environ Monit Assess       Date:  2015-08-21       Impact factor: 2.513

4.  Isotopic evidence for large gaseous nitrogen losses from tropical rainforests.

Authors:  Benjamin Z Houlton; Daniel M Sigman; Lars O Hedin
Journal:  Proc Natl Acad Sci U S A       Date:  2006-05-25       Impact factor: 11.205

5.  A climate-driven switch in plant nitrogen acquisition within tropical forest communities.

Authors:  Benjamin Z Houlton; Daniel M Sigman; Edward A G Schuur; Lars O Hedin
Journal:  Proc Natl Acad Sci U S A       Date:  2007-05-14       Impact factor: 11.205

6.  Changes in North Atlantic nitrogen fixation controlled by ocean circulation.

Authors:  Marietta Straub; Daniel M Sigman; Haojia Ren; Alfredo Martínez-García; A Nele Meckler; Mathis P Hain; Gerald H Haug
Journal:  Nature       Date:  2013-08-21       Impact factor: 49.962

7.  Hadal biosphere: insight into the microbial ecosystem in the deepest ocean on Earth.

Authors:  Takuro Nunoura; Yoshihiro Takaki; Miho Hirai; Shigeru Shimamura; Akiko Makabe; Osamu Koide; Tohru Kikuchi; Junichi Miyazaki; Keisuke Koba; Naohiro Yoshida; Michinari Sunamura; Ken Takai
Journal:  Proc Natl Acad Sci U S A       Date:  2015-02-23       Impact factor: 11.205

8.  An oligotrophic deep-subsurface community dependent on syntrophy is dominated by sulfur-driven autotrophic denitrifiers.

Authors:  Maggie C Y Lau; Thomas L Kieft; Olukayode Kuloyo; Borja Linage-Alvarez; Esta van Heerden; Melody R Lindsay; Cara Magnabosco; Wei Wang; Jessica B Wiggins; Ling Guo; David H Perlman; Saw Kyin; Henry H Shwe; Rachel L Harris; Youmi Oh; Min Joo Yi; Roland Purtschert; Greg F Slater; Shuhei Ono; Siwen Wei; Long Li; Barbara Sherwood Lollar; Tullis C Onstott
Journal:  Proc Natl Acad Sci U S A       Date:  2016-11-21       Impact factor: 11.205

9.  Natural forcing of the North Atlantic nitrogen cycle in the Anthropocene.

Authors:  Xingchen Tony Wang; Anne L Cohen; Victoria Luu; Haojia Ren; Zhan Su; Gerald H Haug; Daniel M Sigman
Journal:  Proc Natl Acad Sci U S A       Date:  2018-10-01       Impact factor: 11.205

10.  Diverse, uncultivated bacteria and archaea underlying the cycling of dissolved protein in the ocean.

Authors:  William D Orsi; Jason M Smith; Shuting Liu; Zhanfei Liu; Carole M Sakamoto; Susanne Wilken; Camille Poirier; Thomas A Richards; Patrick J Keeling; Alexandra Z Worden; Alyson E Santoro
Journal:  ISME J       Date:  2016-03-08       Impact factor: 10.302
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