D Henderson1, T Wilson. 1. Department of Obstetrics and Gynaecology, Dundee University and Medical School, Scotland.
Abstract
OBJECTIVE: There is indirect evidence of decreased progesterone-activated transcription after human labor onset. Binding of the progesterone receptor to its response element is a prerequisite of progesterone-activated transcription. We established an assay to investigate whether there is reduced binding of progesterone receptor to its nuclear response element after, compared with before, labor onset. STUDY DESIGN: The binding of progesterone receptor from the decidua to its nuclear response element was measured in gel shift assays. Tissues from 52 patients who were term, preterm, in labor, and not in labor were compared. RESULTS: A 9-fold decrease in progesterone receptor binding to its response element was observed in tissues obtained after, compared with before, the onset of labor (P = .0008). In both preterm and term not-in-labor tissues, binding was higher than for in-labor tissues (P = .0172 for preterm; P = .0147 for term, Mann-Whitney U test). CONCLUSION: These findings provide a mechanism for the effective withdrawal of progesterone in human parturition.
OBJECTIVE: There is indirect evidence of decreased progesterone-activated transcription after humanlabor onset. Binding of the progesterone receptor to its response element is a prerequisite of progesterone-activated transcription. We established an assay to investigate whether there is reduced binding of progesterone receptor to its nuclear response element after, compared with before, labor onset. STUDY DESIGN: The binding of progesterone receptor from the decidua to its nuclear response element was measured in gel shift assays. Tissues from 52 patients who were term, preterm, in labor, and not in labor were compared. RESULTS: A 9-fold decrease in progesterone receptor binding to its response element was observed in tissues obtained after, compared with before, the onset of labor (P = .0008). In both preterm and term not-in-labor tissues, binding was higher than for in-labor tissues (P = .0172 for preterm; P = .0147 for term, Mann-Whitney U test). CONCLUSION: These findings provide a mechanism for the effective withdrawal of progesterone in human parturition.
Authors: Roberto Romero; Kypros Nicolaides; Agustin Conde-Agudelo; Ann Tabor; John M O'Brien; Elcin Cetingoz; Eduardo Da Fonseca; George W Creasy; Katharina Klein; Line Rode; Priya Soma-Pillay; Shalini Fusey; Cetin Cam; Zarko Alfirevic; Sonia S Hassan Journal: Am J Obstet Gynecol Date: 2011-12-11 Impact factor: 8.661
Authors: JoonHo Lee; Roberto Romero; Yi Xu; Jezid Miranda; Wonsuk Yoo; Piya Chaemsaithong; Juan Pedro Kusanovic; Tinnakorn Chaiworapongsa; Adi L Tarca; Steven J Korzeniewski; Sonia S Hassan; Nandor Gabor Than; Bo Hyun Yoon; Chong Jai Kim Journal: Am J Reprod Immunol Date: 2013-08 Impact factor: 3.886
Authors: Roberto Romero; Agustin Conde-Agudelo; Eduardo Da Fonseca; John M O'Brien; Elcin Cetingoz; George W Creasy; Sonia S Hassan; Kypros H Nicolaides Journal: Am J Obstet Gynecol Date: 2017-11-17 Impact factor: 8.661