OBJECTIVE: Tumors arising from different sites of the head and neck area have different clinical behavior. However, most of the studies on genetic alterations in head and neck squamous cell carcinomas do not make a distinction between the sites within this area. The objective of this study is to compare the genetic alterations in three different sites of the head and neck (larynx, oropharynx, and hypopharynx). STUDY DESIGN: Prospective study. METHODS: Thirty-eight laryngeal, 29 oropharyngeal, and 37 hypopharyngeal carcinomas were studied. DNA from tumor and healthy tissue was evaluated for amplification of the oncogenes at 11q13 region (CCND1, FGF3, FGF4 and EMS1) and of the oncogenes MYC and ERBB1; for integration of the human papillomavirus (HPV) types 6b and 16; for loss of heterozygosity (LOH) at p53 and NAT2; and for the cellular DNA content. RESULTS: FGF3 and FGF4 showed a significantly higher frequency of amplification in hypopharyngeal tumors (P =.006 and P =.0002, respectively). CCND1 amplification had a nearly statistically significant (P =.072) higher frequency of amplification in hypopharyngeal tumors. Aneuploid tumors were found in a significantly lower proportion in the larynx (P =.03) compared with the other sites. For the other genetic alterations, no significant differences among the three sites were found. CONCLUSIONS: These results suggest that cancers originating from different sites in the head and neck may have different tumor biology. Therefore, they should be considered as different entities.
OBJECTIVE:Tumors arising from different sites of the head and neck area have different clinical behavior. However, most of the studies on genetic alterations in head and neck squamous cell carcinomas do not make a distinction between the sites within this area. The objective of this study is to compare the genetic alterations in three different sites of the head and neck (larynx, oropharynx, and hypopharynx). STUDY DESIGN: Prospective study. METHODS: Thirty-eight laryngeal, 29 oropharyngeal, and 37 hypopharyngeal carcinomas were studied. DNA from tumor and healthy tissue was evaluated for amplification of the oncogenes at 11q13 region (CCND1, FGF3, FGF4 and EMS1) and of the oncogenes MYC and ERBB1; for integration of the human papillomavirus (HPV) types 6b and 16; for loss of heterozygosity (LOH) at p53 and NAT2; and for the cellular DNA content. RESULTS:FGF3 and FGF4 showed a significantly higher frequency of amplification in hypopharyngeal tumors (P =.006 and P =.0002, respectively). CCND1 amplification had a nearly statistically significant (P =.072) higher frequency of amplification in hypopharyngeal tumors. Aneuploid tumors were found in a significantly lower proportion in the larynx (P =.03) compared with the other sites. For the other genetic alterations, no significant differences among the three sites were found. CONCLUSIONS: These results suggest that cancers originating from different sites in the head and neck may have different tumor biology. Therefore, they should be considered as different entities.
Authors: Julie C Nienstedt; Alexander Gröbe; Patrick Lebok; Franziska Büscheck; Till Clauditz; Ronald Simon; Asmus Heumann; Guido Sauter; Christoph Moebius; Adrian Münscher; Rainald Knecht; Marco Blessmann; Max Heiland; Christina Pflug Journal: Clin Oral Investig Date: 2016-07-21 Impact factor: 3.573
Authors: Angela E Zou; Jonjei Ku; Thomas K Honda; Vicky Yu; Selena Z Kuo; Hao Zheng; Yinan Xuan; Maarouf A Saad; Andrew Hinton; Kevin T Brumund; Jonathan H Lin; Jessica Wang-Rodriguez; Weg M Ongkeko Journal: RNA Date: 2015-04-22 Impact factor: 4.942
Authors: Rob Noorlag; Pauline M W van Kempen; Inge Stegeman; Ron Koole; Robert J J van Es; Stefan M Willems Journal: Virchows Arch Date: 2015-02-07 Impact factor: 4.064
Authors: Maria J Worsham; Mei Lu; Kang Mei Chen; Josena K Stephen; Shaleta Havard; Vanessa P Schweitzer Journal: J Oncol Date: 2012-04-12 Impact factor: 4.375