Literature DB >> 11566362

Mouse nestin cDNA cloning and protein expression in the cytoskeleton of transfected cells.

J Yang1, L Cheng, Y Yan, W Bian, Y Tomooka, R Shiurba, N Jing.   

Abstract

Complementary DNA (cDNA) corresponding to mouse nestin intermediate filament protein, a specific marker for neural stem cells, was isolated and characterized. The complete sequence comprised 5983 base pairs encoding 1821 amino acids, and the deduced polypeptide was similar to rat (84%), hamster (73%), and human (62%) nestin. Southern blots showed that mouse nestin was a single-copy gene, and Northern blots detected a 6.0 kilobase mRNA transcript. When the cDNA was overexpressed as an enhanced green fluorescent fusion protein in COS7 cells, nestin immunoreactivity appeared in the filamentous cytoskeletal network. Accordingly, biologically active mouse nestin cDNA may offer an important new tool for stem cell research.

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Year:  2001        PMID： 11566362     DOI： 10.1016/s0167-4781(01)00275-5

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  7 in total

1.  Nestin promotes the phosphorylation-dependent disassembly of vimentin intermediate filaments during mitosis.

Authors:  Ying-Hao Chou; Satya Khuon; Harald Herrmann; Robert D Goldman
Journal:  Mol Biol Cell       Date:  2003-04       Impact factor: 4.138

Review 2.  Adult neurogenesis and cellular brain repair with neural progenitors, precursors and stem cells.

Authors:  U Shivraj Sohur; Jason G Emsley; Bartley D Mitchell; Jeffrey D Macklis
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  2006-09-29       Impact factor: 6.237

3.  Development of neural stem cells at different sites of fetus brain of different gestational age.

Authors:  Xiaojuan Yin; Lihua Li; Xiaoying Zhang; Yao Yang; Yannan Chai; Xiao Han; Zhichun Feng
Journal:  Int J Clin Exp Pathol       Date:  2013-11-15

4.  Nestin expression correlates with nerve and retroperitoneal tissue invasion in pancreatic cancer.

Authors:  Masao Kawamoto; Toshiyuki Ishiwata; Kazumitsu Cho; Eiji Uchida; Murray Korc; Zenya Naito; Takashi Tajiri
Journal:  Hum Pathol       Date:  2008-09-16       Impact factor: 3.466

5.  Different transcription factors regulate nestin gene expression during P19 cell neural differentiation and central nervous system development.

Authors:  Zhigang Jin; Li Liu; Wei Bian; Yongfeng Chen; Guoliang Xu; Leping Cheng; Naihe Jing
Journal:  J Biol Chem       Date:  2009-01-15       Impact factor: 5.157

6.  Nestin modulates glucocorticoid receptor function by cytoplasmic anchoring.

Authors:  Rudolph Reimer; Heike Helmbold; Beata Szalay; Christian Hagel; Heinrich Hohenberg; Wolfgang Deppert; Wolfgang Bohn
Journal:  PLoS One       Date:  2009-06-29       Impact factor: 3.240

7.  Expression of the zebrafish intermediate neurofilament Nestin in the developing nervous system and in neural proliferation zones at postembryonic stages.

Authors:  Julia Mahler; Wolfgang Driever
Journal:  BMC Dev Biol       Date:  2007-07-25       Impact factor: 1.978
  7 in total

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