Literature DB >> 11566183

Functional reconstitution of Arabidopsis thaliana plant uncoupling mitochondrial protein (AtPUMP1) expressed in Escherichia coli.

J Borecký1, I G Maia, A D Costa, P Jezek, H Chaimovich, P B de Andrade, A E Vercesi, P Arruda.   

Abstract

The Arabidopsis thaliana uncoupling protein (UCP) gene was expressed in Escherichia coli and isolated protein reconstituted into liposomes. Linoleic acid-induced H+ fluxes were sensitive to purine nucleotide inhibition with an apparent K(i) (in mM) of 0.8 (GDP), 0.85 (ATP), 0.98 (GTP), and 1.41 (ADP); the inhibition was pH-dependent. Kinetics of AtPUMP1-mediated H+ fluxes were determined for lauric, myristic, palmitic, oleic, linoleic, and linolenic acids. Properties of recombinant AtPUMP1 indicate that it represents a plant counterpart of animal UCP2 or UCP3. This work brings the functional and genetic approaches together for the first time, providing strong support that AtPUMP1 is truly an UCP.

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Year:  2001        PMID： 11566183     DOI： 10.1016/s0014-5793(01)02835-6

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  13 in total

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3.  Overexpression of plant uncoupling mitochondrial protein in transgenic tobacco increases tolerance to oxidative stress.

Authors:  Marcos Brandalise; Ivan G Maia; Jiri Borecký; Aníbal E Vercesi; Paulo Arruda
Journal:  J Bioenerg Biomembr       Date:  2003-06       Impact factor: 2.945

4.  Purification and characterization of the reconstitutively active adenine nucleotide carrier from mitochondria of Jerusalem artichoke (Helianthus tuberosus L.) tubers.

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5.  Hydrogen sulfide inhibits the growth of Escherichia coli through oxidative damage.

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6.  Uncoupling proteins 1 and 2 (UCP1 and UCP2) from Arabidopsis thaliana are mitochondrial transporters of aspartate, glutamate, and dicarboxylates.

Authors:  Magnus Monné; Lucia Daddabbo; David Gagneul; Toshihiro Obata; Björn Hielscher; Luigi Palmieri; Daniela Valeria Miniero; Alisdair R Fernie; Andreas P M Weber; Ferdinando Palmieri
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9.  AhDGR2, an amaranth abiotic stress-induced DUF642 protein gene, modifies cell wall structure and composition and causes salt and ABA hyper-sensibility in transgenic Arabidopsis.

Authors:  Paola A Palmeros-Suárez; Julio A Massange-Sánchez; Lino Sánchez-Segura; Norma A Martínez-Gallardo; Eduardo Espitia Rangel; Juan F Gómez-Leyva; John P Délano-Frier
Journal:  Planta       Date:  2016-12-17       Impact factor: 4.116

10.  Mitochondrial uncoupling protein is required for efficient photosynthesis.

Authors:  Lee J Sweetlove; Anna Lytovchenko; Megan Morgan; Adriano Nunes-Nesi; Nicolas L Taylor; Charles J Baxter; Ira Eickmeier; Alisdair R Fernie
Journal:  Proc Natl Acad Sci U S A       Date:  2006-12-05       Impact factor: 11.205
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