Cell migration and DNA synthesis in organ culture of human skin.

Cell migration and DNA synthesis in organ cultured skin were studied. Cell migration leads to the formation of a new epithelial layer around the free edges of the dermis. No significant DNA synthesis occurs in this newly formed epithelium. In contrast, DNA synthesis in the original epidermis is maintained throughout the period of culture as shown by the uptake of radioactive thymidine. Addition of hydrocortisone to the culture medium increases cell migration, and phytohaemagglutinin, while dramatically stimulating mitotic activity, suppresses cell migration. These results show that an increase in epithelial cell division is accompanied by diminished cell migration whereas an increase in cell migration occurs in the presence of a decrease in epithelial cell division. The behavaiour of skin in organ culture during the first 48-72 h can be compared with that seen in wound healing.