| Literature DB >> 11563067 |
N Polushin1, A Malykh, O Malykh, M Zenkova, N Chumakova, V Vlassov, S Kozyavkin.
Abstract
Synthesis of 2'-modified oligonucleotides from 2'-methoxyoxalamido (MOX) and 2'-succinimido (SUC) precursors is described. Their physical and biochemical properties were assessed. Synthesized oligonucleotides were used as primers in advanced DNA sequencing protocols. An example of sequencing directly off genomic DNA template without prior cloning or PCR amplification is presented.Entities:
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Year: 2001 PMID： 11563067 DOI： 10.1081/NCN-100002326
Source DB: PubMed Journal: Nucleosides Nucleotides Nucleic Acids ISSN： 1525-7770 Impact factor: 1.381