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Literature DB >> 11563066

Analogs of guanine nucleoside triphosphates for sequencing applications.

M G McDougall¹, L Sun, I Livshin, L P Hosta, B F McArdle, S B Samols, C W Fuller, S Kumar.

Abstract

We have synthesized more than 30 different deoxyribonucleosides and triphosphates with modifications either in the base or the phosphate moiety as analogs of 2'-dGTP for DNA sequencing applications. All the modified nucleoside triphosphates were tested as substrates for DNA polymerases, including Sequenase T7 DNA polymerase or Thermo Sequenase DNA polymerase. Two of the analogs, 7-ethyl-7-deaza-dGTP and 7-hydroxymethyl-7-deaza-dGTP meet our requirements as better sequencing reagents.

Entities: Chemical

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Year: 2001 PMID： 11563066 DOI： 10.1081/NCN-100002325

Source DB: PubMed Journal: Nucleosides Nucleotides Nucleic Acids ISSN： 1525-7770 Impact factor: 1.381

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Cited

6 in total

1. Synthesis and application of charge-modified dye-labeled dideoxynucleoside-5'-triphosphates to 'direct-load' DNA sequencing.

Authors: Patrick J Finn; Lei Sun; Satyam Nampalli; Haiguang Xiao; John R Nelson; J Anthony Mamone; Greg Grossmann; Parke K Flick; Carl W Fuller; Shiv Kumar
Journal: Nucleic Acids Res Date: 2002-07-01 Impact factor: 16.971

2. Stereochemistry of benzylic carbon substitution coupled with ring modification of 2-nitrobenzyl groups as key determinants for fast-cleaving reversible terminators.

Authors: Brian P Stupi; Hong Li; Jinchun Wang; Weidong Wu; Sidney E Morris; Vladislav A Litosh; Jesse Muniz; Megan N Hersh; Michael L Metzker
Journal: Angew Chem Int Ed Engl Date: 2012-01-09 Impact factor: 15.336

Analogs of guanine nucleoside triphosphates for sequencing applications.

1. Synthesis and application of charge-modified dye-labeled dideoxynucleoside-5'-triphosphates to 'direct-load' DNA sequencing.

2. Stereochemistry of benzylic carbon substitution coupled with ring modification of 2-nitrobenzyl groups as key determinants for fast-cleaving reversible terminators.

3. Enzymatic synthesis of structure-free DNA with pseudo-complementary properties.

Review 4. Applications of Ruthenium Complexes Covalently Linked to Nucleic Acid Derivatives.

5. Properties of pseudo-complementary DNA substituted with weakly pairing analogs of guanine or cytosine.

6. Artificially expanded genetic information system: a new base pair with an alternative hydrogen bonding pattern.