Storage and sexual separation of Glossina morsitans morsitans Westwood puparia.

Maintenance of unknown-age tsetse puparia at 4 degrees C for six days substantially reduced emergence; normal emergence occurred when puparia were held at temperatures of 12 degrees, 14 degrees, or 16 degrees C for two, three or four weeks, respectively. In-storage eclosion of adults was either suppressed completely at the temperatures used or was slight. Weight, puparial duration, and density of puparia were examined in an attempt to detect sexual differences in puparia. There appeared to be no usable correlation between pupal weight and sex, but the difference between the puparial duration of each sex offers a mechanism for retaining 81% of the females produced and releasing 84% of the males. The flotation of unknown-or known-age puparia in methanol-water solutions of varying specific gravities demonstrated some diffences in the density of male and female puparia. However, these differences were not great or consistent enough to provide an efficient system of separating puparia by age or sex.