Literature DB >> 11556407

Identification and molecular characterisation of the complete genome of a Singapore isolate of hepatitis C virus: sequence comparison with other strains and phylogenetic analysis.

S P Lim1, Y L Khu, W J Hong, A Tay, A E Ting, S G Lim, Y H Tan.   

Abstract

The complete genomic sequence of a Singapore isolate of the hepatitis C virus (HCV) was obtained from serum of an individual chronically infected with HCV. Nine overlapping cDNA clones covering the entire viral genome was amplified by reverse-transcription-polymerase chain reaction (RT-PCR), This isolate (HCV-S1) comprised 9,609 nucleotides (nt), including 341 nt of the complete 5' untranslated region (5' UTR), a single open reading frame of 3,011 amino acids (aa) and 235 nt of the complete 3' UTR. Its genotype was identified as type lb from analyses of its sequences in the 5' UTR, NS3 and NS5B regions. When compared against nine reported HCV isolates, the overall aa homology of HCV-SI was closest with an Australian strain, HCV-A (94%) and a Japanese strain, HCV-JT (93.9%). Phylogenetic analysis revealed that it was most closely related to the Taiwan strain, HCV-TW and another Japanese strain, HCV-K1-R1.

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Year:  2001        PMID： 11556407     DOI： 10.1023/a:1011143731677

Source DB:  PubMed          Journal:  Virus Genes        ISSN： 0920-8569            Impact factor:   2.332


  29 in total

1.  Nucleotide sequence of the genomic RNA of hepatitis C virus isolated from a human carrier: comparison with reported isolates for conserved and divergent regions.

Authors:  H Okamoto; S Okada; Y Sugiyama; K Kurai; H Iizuka; A Machida; Y Miyakawa; M Mayumi
Journal:  J Gen Virol       Date:  1991-11       Impact factor: 3.891

2.  Molecular cloning of the human hepatitis C virus genome from Japanese patients with non-A, non-B hepatitis.

Authors:  N Kato; M Hijikata; Y Ootsuyama; M Nakagawa; S Ohkoshi; T Sugimura; K Shimotohno
Journal:  Proc Natl Acad Sci U S A       Date:  1990-12       Impact factor: 11.205

3.  Structure and organization of the hepatitis C virus genome isolated from human carriers.

Authors:  A Takamizawa; C Mori; I Fuke; S Manabe; S Murakami; J Fujita; E Onishi; T Andoh; I Yoshida; H Okayama
Journal:  J Virol       Date:  1991-03       Impact factor: 5.103

4.  Identification of a highly conserved sequence element at the 3' terminus of hepatitis C virus genome RNA.

Authors:  A A Kolykhalov; S M Feinstone; C M Rice
Journal:  J Virol       Date:  1996-06       Impact factor: 5.103

5.  Pretreatment serum hepatitis C virus RNA levels and hepatitis C virus genotype are the main and independent prognostic factors of sustained response to interferon alfa therapy in chronic hepatitis C.

Authors:  M Martinot-Peignoux; P Marcellin; M Pouteau; C Castelnau; N Boyer; M Poliquin; C Degott; I Descombes; V Le Breton; V Milotova
Journal:  Hepatology       Date:  1995-10       Impact factor: 17.425

6.  Prevalence, genotypes, and an isolate (HC-C2) of hepatitis C virus in Chinese patients with liver disease.

Authors:  Y Wang; H Okamoto; F Tsuda; R Nagayama; Q M Tao; S Mishiro
Journal:  J Med Virol       Date:  1993-07       Impact factor: 2.327

7.  Prevalence of hepatitis C virus sequence variants in South-East Asia.

Authors:  W K Greene; M K Cheong; V Ng; K W Yap
Journal:  J Gen Virol       Date:  1995-01       Impact factor: 3.891

8.  Mutations in the nonstructural protein 5A gene and response to interferon in patients with chronic hepatitis C virus 1b infection.

Authors:  N Enomoto; I Sakuma; Y Asahina; M Kurosaki; T Murakami; C Yamamoto; Y Ogura; N Izumi; F Marumo; C Sato
Journal:  N Engl J Med       Date:  1996-01-11       Impact factor: 91.245

9.  A novel sequence found at the 3' terminus of hepatitis C virus genome.

Authors:  T Tanaka; N Kato; M J Cho; K Shimotohno
Journal:  Biochem Biophys Res Commun       Date:  1995-10-13       Impact factor: 3.575

10.  Hepatitis C virus (HCV) genotype distribution in German isolates: studies on the sequence variability in the E2 and NS5 region.

Authors:  G Driesel; D Wirth; K Stark; R Baumgarten; U Sucker; E Schreier
Journal:  Arch Virol       Date:  1994       Impact factor: 2.574
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  5 in total

1.  Responses of nontransformed human hepatocytes to conditional expression of full-length hepatitis C virus open reading frame.

Authors:  Weiliang Tang; Catherine A Lázaro; Jean S Campbell; W Tony Parks; Michael G Katze; Nelson Fausto
Journal:  Am J Pathol       Date:  2007-11-08       Impact factor: 4.307

2.  Hepatitis C virus non-structural protein NS3 interacts with LMP7, a component of the immunoproteasome, and affects its proteasome activity.

Authors:  Yee-Ling Khu; Yee-Joo Tan; Seng Gee Lim; Wanjin Hong; Phuay-Yee Goh
Journal:  Biochem J       Date:  2004-12-01       Impact factor: 3.857

3.  Intrinsically unstructured domain 3 of hepatitis C Virus NS5A forms a "fuzzy complex" with VAPB-MSP domain which carries ALS-causing mutations.

Authors:  Garvita Gupta; Haina Qin; Jianxing Song
Journal:  PLoS One       Date:  2012-06-13       Impact factor: 3.240

4.  Cellular RNA helicase p68 relocalization and interaction with the hepatitis C virus (HCV) NS5B protein and the potential role of p68 in HCV RNA replication.

Authors:  Phuay-Yee Goh; Yee-Joo Tan; Siew Pheng Lim; Y H Tan; Seng Gee Lim; Frances Fuller-Pace; Wanjin Hong
Journal:  J Virol       Date:  2004-05       Impact factor: 5.103

5.  The use of hepatitis C virus NS3/4A and secreted alkaline phosphatase to quantitate cell-cell membrane fusion mediated by severe acute respiratory syndrome coronavirus S protein and the receptor angiotensin-converting enzyme 2.

Authors:  Chih-Fong Chou; Shuo Shen; Geetha Mahadevappa; Seng Gee Lim; Wanjin Hong; Yee-Joo Tan
Journal:  Anal Biochem       Date:  2007-04-27       Impact factor: 3.365
  5 in total

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