H Sato1, S Shiobara, S Yasue, T Chuhjo, S Nakao. 1. Division of Transfusion Medicine, Kanazawa University Hospital, Kanazawa, Ishikawa, Japan. hsatoh@med.kanazawa-u.ac.jp
Abstract
BACKGROUND AND OBJECTIVES: To estimate the minimum volume of processed blood necessary for the purpose of donor leucocyte infusion (DLI), we determined the number of CD3+ cells harvested by apheresis from normal donors and examined adverse events during the procedure. MATERIALS AND METHODS: Leukapheresis utilizing the COBE Spectra' was performed a total of 24 times from 12 normal donors. Blood counts were obtained and the number of CD3+ cells was evaluated before and after apheresis and in the peripheral blood products. Complications associated with the procedure were documented. RESULTS: Blood products contained, on average, 51.1% CD3+ cells. A linear correlation was found between the number of CD3+ cells collected and the processed blood volume, up to 12 000 ml (r = 0.930, P < 0.001). Cytoreduction was observed in all donors after apheresis. In particular, the platelet and lymphocyte values decreased to approximately 70% of the preapheresis value. Two donors reported adverse effects: one was a mild vaso-vagal reaction and the other was citrate-related lip paresthesia. These symptoms were mild and disappeared spontaneously. CONCLUSIONS: We found a linear correlation between the number of CD3+ cells collected and the processed blood volume, which enabled us to estimate the minimum volume of processed blood necessary for DLI. The adverse events observed were acceptable.
BACKGROUND AND OBJECTIVES: To estimate the minimum volume of processed blood necessary for the purpose of donor leucocyte infusion (DLI), we determined the number of CD3+ cells harvested by apheresis from normal donors and examined adverse events during the procedure. MATERIALS AND METHODS: Leukapheresis utilizing the COBE Spectra' was performed a total of 24 times from 12 normal donors. Blood counts were obtained and the number of CD3+ cells was evaluated before and after apheresis and in the peripheral blood products. Complications associated with the procedure were documented. RESULTS: Blood products contained, on average, 51.1% CD3+ cells. A linear correlation was found between the number of CD3+ cells collected and the processed blood volume, up to 12 000 ml (r = 0.930, P < 0.001). Cytoreduction was observed in all donors after apheresis. In particular, the platelet and lymphocyte values decreased to approximately 70% of the preapheresis value. Two donors reported adverse effects: one was a mild vaso-vagal reaction and the other was citrate-related lip paresthesia. These symptoms were mild and disappeared spontaneously. CONCLUSIONS: We found a linear correlation between the number of CD3+ cells collected and the processed blood volume, which enabled us to estimate the minimum volume of processed blood necessary for DLI. The adverse events observed were acceptable.
Authors: Juhua Zhou; Hina Chaudhry; Yin Zhong; Mir Mustafa Ali; Linda A Perkins; William B Owens; Juan E Morales; Franklin R McGuire; Elizabeth E Zumbrun; Jiajia Zhang; Prakash S Nagarkatti; Mitzi Nagarkatti Journal: Cytokine Date: 2014-09-29 Impact factor: 3.861
Authors: Ran Reshef; Austin P Huffman; Amy Gao; Marlise R Luskin; Noelle V Frey; Saar I Gill; Elizabeth O Hexner; Taku Kambayashi; Alison W Loren; Selina M Luger; James K Mangan; Sunita D Nasta; Lee P Richman; Mary Sell; Edward A Stadtmauer; Robert H Vonderheide; Rosemarie Mick; David L Porter Journal: J Clin Oncol Date: 2015-06-08 Impact factor: 44.544