Literature DB >> 11549456

Chemo-enzymatic synthesis of the Galili epitope Gal(alpha)(1-->3)Galbeta(1-->4)GlcNAc on a homogeneously soluble PEG polymer by a multi-enzyme system.

N Brinkmann1, M Malissard, M Ramuz, U Römer, T Schumacher, E G Berger, L Elling, C Wandrey, A Liese.   

Abstract

The alpha-Gal trisaccharide Gal(alpha)(1-->3)Galbeta(1-->4)GlcNAc 11 was synthesized on a homogeneously soluble polymeric support (polyethylene glycol, PEG) by use of a multi-enzyme system consisting of beta-1,4-galactosyltransferase (EC 2.4.1.38), alpha-1,3-galactosyltransferase (EC 2.4.1.151), sucrose synthase (EC 2.4.1.13) and UDP-glucose-4-epimerase (EC 5.1.3.2). In addition workup was simplified by use of dia-ultrafiltration. Thus the advantages of classic chemistry/enzymology and solid-phase synthesis could be united in one. Subsequent hydrogenolytic cleavage afforded the free alpha-Gal trisaccharide.

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Year:  2001        PMID： 11549456     DOI： 10.1016/s0960-894x(01)00474-7

Source DB:  PubMed          Journal:  Bioorg Med Chem Lett        ISSN： 0960-894X            Impact factor:   2.823


  4 in total

1.  P1 Trisaccharide (Galalpha1,4Galbeta1,4GlcNAc) synthesis by enzyme glycosylation reactions using recombinant Escherichia coli.

Authors:  Ziye Liu; Yuquan Lu; Jianbo Zhang; Keith Pardee; Peng George Wang
Journal:  Appl Environ Microbiol       Date:  2003-04       Impact factor: 4.792

2.  Synthesis of Galα(1,3)Galβ(1,4)GlcNAcα-, Galβ(1,4)GlcNAcα- and GlcNAc-containing neoglycoproteins and their immunological evaluation in the context of Chagas disease.

Authors:  Nathaniel S Schocker; Susana Portillo; Carlos R N Brito; Alexandre F Marques; Igor C Almeida; Katja Michael
Journal:  Glycobiology       Date:  2015-09-18       Impact factor: 4.313

3.  Chemo-enzymatic synthesis of poly-N-acetyllactosamine (poly-LacNAc) structures and their characterization for CGL2-galectin-mediated binding of ECM glycoproteins to biomaterial surfaces.

Authors:  Birgit Sauerzapfe; Karel Krenek; Judith Schmiedel; Warren W Wakarchuk; Helena Pelantová; Vladimir Kren; Lothar Elling
Journal:  Glycoconj J       Date:  2008-08-29       Impact factor: 2.916

4.  Chemo-enzymatic modification of poly-N-acetyllactosamine (LacNAc) oligomers and N,N-diacetyllactosamine (LacDiNAc) based on galactose oxidase treatment.

Authors:  Christiane E Kupper; Ruben R Rosencrantz; Birgit Henßen; Helena Pelantová; Stephan Thönes; Anna Drozdová; Vladimir Křen; Lothar Elling
Journal:  Beilstein J Org Chem       Date:  2012-05-09       Impact factor: 2.883
  4 in total

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