Influence of water activity and temperature on the growth of Wallemia sebi: application of a predictive model.

Germination and growth of Wallemia sebi were examined on media of a(w) adjusted with glycerol in the range of 0.96-0.77, at 25 degrees C and 30 degrees C. The effect of temperature on the germination time was significant except between 0.95 and 0.88 a(w). At low a(w) levels as well as above 0.95, the increase of temperature produced an increment in the germination time. The minimum a(w) for germination was also affected by temperature, being lower at 25 degrees C (0.80 a(w)) than at 30 degrees C (0.82 a(w)). Radial growth rates at 25 degrees C were higher than at 30 degrees C. The optimum a(w) value for growth of W. sebi was 0.94 at both temperatures. The minimum a(w) for growth was higher than minimum for germination and was also dependent on temperature (0.84 at 25 degrees C and 0.86 at 30 degrees C). An empirical mathematical model was fitted to the measured growth data, providing a good approach to the description of the effect of a(w) on the radial growth rate of W. sebi.