| Literature DB >> 11541102 |
R P Schwarz1, T J Goodwin, D A Wolf.
Abstract
High-density, three-dimensional cell cultures are difficult to grow in vitro. The rotating-wall vessel (RWV) described here has cultured BHK-21 cells to a density of 1.1 X 10(7) cells/ml. Cells on microcarriers were observed to grow with enhanced bridging in this batch culture system. The RWV is a horizontally rotated tissue culture vessel with silicon membrane oxygenation. This design results in a low-turbulence, low-shear cell culture environment with abundant oxygenation. The RWV has the potential to culture a wide variety of normal and neoplastic cells.Entities:
Mesh：
Year: 1992 PMID： 11541102 DOI： 10.1007/bf01404744
Source DB: PubMed Journal: J Tissue Cult Methods ISSN： 0271-8057