| Literature DB >> 11538270 |
Abstract
An electron-microscopic cytochemical method was used to localize diamine oxidase (DAO) in pea and polyamine oxidase (PAO) in maize (Zea mays L.). The method, based on the precipitation of amine-oxidase-generated H2O2 by CeCl3, was shown to be specific for DAO and PAO and permitted their localization in plant tissues with a high degree of resolution. Both enzymes are localized exclusively in the cell wall. Both DAO- and PAO-activity staining is most intense in the middle lamellar region of the wall and in cells exhibiting highly lignified walls. The oxidases could provide H2O2 for peroxidase-mediated cross-linking reactions in the cell wall and may, in this capacity, play a role in the regulation of plant growth.Entities:
Keywords: NASA Discipline Number 40-50; NASA Discipline Plant Biology; NASA Program Space Biology; Non-NASA Center
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Year: 1991 PMID： 11538270 DOI： 10.1007/bf00197744
Source DB: PubMed Journal: Planta ISSN： 0032-0935 Impact factor: 4.116