Literature DB >> 11533190

Rescue of recombinant Thogoto virus from cloned cDNA.

E Wagner1, O G Engelhardt, S Gruber, O Haller, G Kochs.   

Abstract

Thogoto virus (THOV) is a tick-transmitted orthomyxovirus with a genome consisting of six negative-stranded RNA segments. To rescue a recombinant THOV, the viral structural proteins were produced from expression plasmids by means of a vaccinia virus expressing the T7 RNA polymerase. Genomic virus RNAs (vRNAs) were generated from plasmids under the control of the RNA polymerase I promoter. Using this system, we could efficiently recover recombinant THOV following transfection of 12 plasmids into 293T cells. To verify the recombinant nature of the rescued virus, specific genetic tags were introduced into two vRNA segments. The availability of this efficient reverse genetics system will allow us to address hitherto-unanswered questions regarding the biology of THOV by manipulating viral genes in the context of infectious virus.

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Year:  2001        PMID： 11533190      PMCID： PMC114495          DOI： 10.1128/JVI.75.19.9282-9286.2001

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  26 in total

Review 1.  Inhibition of interferon-mediated antiviral responses by influenza A viruses and other negative-strand RNA viruses.

Authors:  A García-Sastre
Journal:  Virology       Date:  2001-01-20       Impact factor: 3.616

2.  A DNA transfection system for generation of influenza A virus from eight plasmids.

Authors:  E Hoffmann; G Neumann; Y Kawaoka; G Hobom; R G Webster
Journal:  Proc Natl Acad Sci U S A       Date:  2000-05-23       Impact factor: 11.205

3.  Isolation of Thogoto virus from Rhipicephalus bursa ticks in western Sicily.

Authors:  M Albanese; C Bruno-Smiraglia; G Di di Cuonzo; A Lavagnino; S Srihongse
Journal:  Acta Virol       Date:  1972-05       Impact factor: 1.162

4.  Tick-borne viruses structurally similar to Orthomyxoviruses.

Authors:  J P Clerx; F Fuller; D H Bishop
Journal:  Virology       Date:  1983-05       Impact factor: 3.616

5.  Thogoto virus matrix protein is encoded by a spliced mRNA.

Authors:  G Kochs; F Weber; S Gruber; A Delvendahl; C Leitz; O Haller
Journal:  J Virol       Date:  2000-11       Impact factor: 5.103

6.  Influenza A virus NS2 protein mediates vRNP nuclear export through NES-independent interaction with hCRM1.

Authors:  G Neumann; M T Hughes; Y Kawaoka
Journal:  EMBO J       Date:  2000-12-15       Impact factor: 11.598

7.  Formation of virus-like particles from cloned cDNAs of Thogoto virus.

Authors:  Elke Wagner; Othmar G Engelhardt; Friedemann Weber; Otto Haller; Georg Kochs
Journal:  J Gen Virol       Date:  2000-12       Impact factor: 3.891

8.  Rescue of influenza A virus from recombinant DNA.

Authors:  E Fodor; L Devenish; O G Engelhardt; P Palese; G G Brownlee; A García-Sastre
Journal:  J Virol       Date:  1999-11       Impact factor: 5.103

9.  The effect of virus-immune hosts on Thogoto virus infection of the tick, Rhipicephalus appendiculatus.

Authors:  L D Jones; P A Nuttall
Journal:  Virus Res       Date:  1989-10       Impact factor: 3.303

10.  The glycoprotein of Thogoto virus (a tick-borne orthomyxo-like virus) is related to the baculovirus glycoprotein GP64.

Authors:  M A Morse; A C Marriott; P A Nuttall
Journal:  Virology       Date:  1992-02       Impact factor: 3.616
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  11 in total

1.  Thogoto virus lacking interferon-antagonistic protein ML is strongly attenuated in newborn Mx1-positive but not Mx1-negative mice.

Authors:  Andreas Pichlmair; Johanna Buse; Stephanie Jennings; Otto Haller; Georg Kochs; Peter Staeheli
Journal:  J Virol       Date:  2004-10       Impact factor: 5.103

2.  RNA polymerase II-controlled expression of antigenomic RNA enhances the rescue efficacies of two different members of the Mononegavirales independently of the site of viral genome replication.

Authors:  Arnold Martin; Peter Staeheli; Urs Schneider
Journal:  J Virol       Date:  2006-06       Impact factor: 5.103

3.  Eight years' advances on Bourbon virus, a tick-born Thogotovirus of the Orthomyxovirus family.

Authors:  Siyuan Hao; Kang Ning; Çağla Aksu Küz; Shane McFarlin; Fang Cheng; Jianming Qiu
Journal:  Zoonoses (Burlingt)       Date:  2022-06-14

4.  Rescue of influenza B virus from eight plasmids.

Authors:  Erich Hoffmann; Kutubuddin Mahmood; Chin-Fen Yang; Robert G Webster; Harry B Greenberg; George Kemble
Journal:  Proc Natl Acad Sci U S A       Date:  2002-08-09       Impact factor: 11.205

5.  The interferon antagonist ML protein of thogoto virus targets general transcription factor IIB.

Authors:  Carola Vogt; Ellen Preuss; Daniel Mayer; Friedemann Weber; Martin Schwemmle; Georg Kochs
Journal:  J Virol       Date:  2008-09-03       Impact factor: 5.103

6.  Novel gene product of Thogoto virus segment 6 codes for an interferon antagonist.

Authors:  Kathrin Hagmaier; Stephanie Jennings; Johanna Buse; Friedemann Weber; Georg Kochs
Journal:  J Virol       Date:  2003-02       Impact factor: 5.103

7.  A mutation on influenza C virus M1 protein affects virion morphology by altering the membrane affinity of the protein.

Authors:  Yasushi Muraki; Toshio Murata; Emi Takashita; Yoko Matsuzaki; Kanetsu Sugawara; Seiji Hongo
Journal:  J Virol       Date:  2007-05-30       Impact factor: 5.103

8.  Rescue of influenza C virus from recombinant DNA.

Authors:  Bernadette Crescenzo-Chaigne; Sylvie van der Werf
Journal:  J Virol       Date:  2007-08-08       Impact factor: 5.103

9.  The cytoplasmic location of chicken mx is not the determining factor for its lack of antiviral activity.

Authors:  Camilla T O Benfield; Jon W Lyall; Laurence S Tiley
Journal:  PLoS One       Date:  2010-08-16       Impact factor: 3.240

Review 10.  Many ways to make an influenza virus--review of influenza virus reverse genetics methods.

Authors:  Othmar G Engelhardt
Journal:  Influenza Other Respir Viruses       Date:  2012-06-19       Impact factor: 4.380
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